Basic information
Locus name	AT5G01820
Alias	CIPK14
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Signal transduction
Effect for Senescence	delay
Gene Description	CIPK24 encodes a CBL-interacting serine/threonine protein kinase.
Evidence	Molecular evidence [Ref 1]
References	1: Ren Y, Li Y, Jiang Y, Wu B, Miao Y. Phosphorylation of WHIRLY1 by CIPK14 Shifts Its_Localization and Dual Functions in Arabidopsis. Mol Plant. 2017 May 1;10(5):749-763. doi: 10.1016/j.molp.2017.03.011. Epub 2017 Apr 12.
Gene Ontology	biological process protein phosphorylation signal transduction molecular function ATP binding protein kinase activity protein serine/threonine kinase activity
Sequence	AT5G01820.1 | Genomic | mRNA | CDS | Protein
Mutant information
Mutated 1 Mutant name oeCIPK14 Mutant/Transgenic transgene Ecotype Col-0 Mutagenesis type transgenic
Mutated 2 Mutant name cipk14 Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type T-DNA
Mutated 3 Mutant name cipk14 Mutant/Transgenic Ecotype Col-0 Mutagenesis type T-DNA
Mutated 4 Mutant name oeCIPK14 Mutant/Transgenic Ecotype Col-0 Mutagenesis type transgene
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT5G01820.1  miRNA: ath-miR1888 miRNA: ath-miR1888 mfe: -20.5 kcal/mol  p-value: 0.057429  position:  1695  target 5' U      G              U 3'             UUC UC ACAAAUCUUAAUUU              AAG AG UGUUUAGAAUUGAA   miRNA  3'     A                 U 5'
Ortholog Group
Ortholog Groups: OG5_212709	Accession Taxon NP_195802 ( AT5G01820 ) Arabidopsis thaliana 27762.m000016 Ricinus communis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR24347 2.1E-185 7 425 IPR020636 Calcium/calmodulin-dependent/calcium-dependent protein kinase SUPERFAMILY SSF56112 1.2E-88 18 305 IPR011009 Protein kinase-like domain Pfam PF00069 9.9E-73 22 276 IPR000719 Protein kinase, catalytic domain ProSiteProfiles PS50011 50.3 22 276 IPR000719 Protein kinase, catalytic domain SMART SM00220 2.0E-102 22 276 IPR002290 Serine/threonine- / dual specificity protein kinase, catalytic domain ProSitePatterns PS00107 NA 28 51 IPR017441 Protein kinase, ATP binding site ProSitePatterns PS00108 NA 140 152 IPR008271 Serine/threonine-protein kinase, active site ProSiteProfiles PS50816 11.7 305 329 IPR018451 NAF/FISL domain Pfam PF03822 2.3E-20 309 366 IPR004041 NAF domain
Mutant Image
Seedlings of a CIPK14 T-DNA insertion line grew yellow from the old leaf to young leaf and died 20 days after germination, showing severe senescence and an early seedling-lethal phenotype.
Subcellular Localization
Localization	cytosol
Evidence	SUBAcon
Pubmed ID	23180787