Basic information
Locus name	AT3G20250
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Others
Effect for Senescence	unclear
Gene Description	pumilio/Puf RNA-binding domain-containing protein contains Pfam profile: PF00806 Pumilio-
Evidence	Genomic evidence:microarray data [Ref 1]
References	1: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85
Gene Ontology	molecular function RNA binding
Sequence	AT3G20250.1 | Genomic | mRNA | CDS | Protein
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT3G20250.1  miRNA: ath-miR1886 miRNA: ath-miR1886 mfe: -19.1 kcal/mol  p-value: 0.400610  position:  197  target 5' U     U G              C 3'             AGUGA A UGGGAUCUUUGGUU              UCAUU U ACUUUAGAAAUUAA   miRNA  3'       C                  5'  target: AT3G20250.1  miRNA: ath-miR2112-5p miRNA: ath-miR2112-5p mfe: -28.6 kcal/mol  p-value: 0.002410  position:  2558  target 5' A       A            U 3'             AUAUUGG AUCUGCGUUUGC              UGUAACU UAGGCGUAAACG   miRNA  3'         A            C 5'
Ortholog Group
Ortholog Groups: OG5_126869	Accession Taxon NP_001031720 Arabidopsis thaliana NP_180478 Arabidopsis thaliana NP_180482 Arabidopsis thaliana NP_180483 Arabidopsis thaliana NP_187647 Arabidopsis thaliana NP_188660 ( AT3G20250 ) Arabidopsis thaliana NP_567733 Arabidopsis thaliana NP_974618 Arabidopsis thaliana CMR410C Cyanidioschyzon merolae strain 10D 123505 Chlamydomonas reinhardtii ACO64984 Micromonas sp. RCC299 NP_001044782 Oryza sativa Japonica Group NP_001045538 Oryza sativa Japonica Group NP_001048533 Oryza sativa Japonica Group NP_001054436 Oryza sativa Japonica Group NP_001062262 Oryza sativa Japonica Group NP_001063558 Oryza sativa Japonica Group NP_001067100 Oryza sativa Japonica Group e_gw1.96.1.1 Physcomitrella patens subsp. patens e_gw1.96.3.1 Physcomitrella patens subsp. patens estExt_Genewise1.C_350182 Physcomitrella patens subsp. patens estExt_Genewise1.C_960051 Physcomitrella patens subsp. patens 29595.m000292 Ricinus communis 30143.m001162 Ricinus communis 30143.m001164 Ricinus communis 30169.m006300 Ricinus communis e_gw1.13.84.1 Thalassiosira pseudonana CCMP1335 e_gw1.2.151.1 Thalassiosira pseudonana CCMP1335 e_gw1.2.252.1 Thalassiosira pseudonana CCMP1335 estExt_thaps1_ua_kg.C_chr_50286 Thalassiosira pseudonana CCMP1335 gw1.7.168.1 Thalassiosira pseudonana CCMP1335 thaps1_ua_kg.chr_5000072 Thalassiosira pseudonana CCMP1335 XP_002958359 Volvox carteri f. nagariensis XP_002959184 Volvox carteri f. nagariensis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR12537 0.0 8 957 No hit NA ProSiteProfiles PS50303 135.7 608 948 IPR001313 Pumilio RNA-binding repeat SUPERFAMILY SSF48371 9.2E-116 610 944 IPR016024 Armadillo-type fold ProSiteProfiles PS50302 10.5 628 663 IPR001313 Pumilio RNA-binding repeat SMART SM00025 2.6E-5 628 663 IPR001313 Pumilio RNA-binding repeat Pfam PF00806 3.9E-6 630 658 IPR001313 Pumilio RNA-binding repeat ProSiteProfiles PS50302 11.4 664 699 IPR001313 Pumilio RNA-binding repeat SMART SM00025 2.7E-9 664 699 IPR001313 Pumilio RNA-binding repeat Pfam PF00806 4.1E-8 667 697 IPR001313 Pumilio RNA-binding repeat ProSiteProfiles PS50302 11.0 700 735 IPR001313 Pumilio RNA-binding repeat SMART SM00025 9.6E-7 700 735 IPR001313 Pumilio RNA-binding repeat Pfam PF00806 3.7E-5 703 732 IPR001313 Pumilio RNA-binding repeat ProSiteProfiles PS50302 8.9 736 771 IPR001313 Pumilio RNA-binding repeat SMART SM00025 9.3E-5 736 771 IPR001313 Pumilio RNA-binding repeat Pfam PF00806 2.8E-6 737 759 IPR001313 Pumilio RNA-binding repeat ProSiteProfiles PS50302 11.0 772 808 IPR001313 Pumilio RNA-binding repeat SMART SM00025 2.4E-8 772 808 IPR001313 Pumilio RNA-binding repeat Pfam PF00806 3.6E-7 774 799 IPR001313 Pumilio RNA-binding repeat ProSiteProfiles PS50302 11.6 809 844 IPR001313 Pumilio RNA-binding repeat SMART SM00025 7.8E-6 809 844 IPR001313 Pumilio RNA-binding repeat Pfam PF00806 7.4E-4 814 843 IPR001313 Pumilio RNA-binding repeat ProSiteProfiles PS50302 11.3 845 880 IPR001313 Pumilio RNA-binding repeat SMART SM00025 2.4E-9 845 880 IPR001313 Pumilio RNA-binding repeat Pfam PF00806 3.5E-9 846 879 IPR001313 Pumilio RNA-binding repeat ProSiteProfiles PS50302 11.3 881 922 IPR001313 Pumilio RNA-binding repeat SMART SM00025 3.7E-8 887 922 IPR001313 Pumilio RNA-binding repeat Pfam PF00806 5.1E-7 889 920 IPR001313 Pumilio RNA-binding repeat
Subcellular Localization
Localization	cytosol,nucleus
Evidence	SUBAcon
Pubmed ID	23180787