Basic information
GenBank ID	AF296077
Alias	DHS
Organism	Tomato (Solanum lycopersicon)
Taxonomic identifier	[NCBI]
Function category	Protein biosynthesis:Translation Initiation
Effect for Senescence	promote
Gene Description	Full-length cDNA clones encoding deoxyhypusine synthase (DHS) and eucaryotic initiation factor 5A (eIF-5A) have been isolated from a cDNA expression library prepared from tomato leaves (Lycopersicon esculentum, cv. Match) exposed to environmental stress. DHS mediates the first of two enzymatic reactions that activate eIF-5A by converting a conserved lysine to the unusual amino acid, deoxyhypusine. Recombinant protein obtained by expressing tomato DHS cDNA in Escherichia coli proved capable of carrying out the deoxyhypusine synthase reaction in vitro in the presence of eIF-5A. Of particular interest is the finding that DHS mRNA and eIF-5A mRNA show a parallel increase in abundance in senescing tomato flowers, senescing tomato fruit, and environmentally stressed tomato leaves exhibiting programmed cell death. Western blot analyses indicated that DHS protein also increases at the onset of senescence.
Evidence	Protein: Western blot [Ref 1]
References	1: Wang TW, Lu L, Wang D, Thompson JE Isolation and characterization of senescence-induced cDNAs encoding deoxyhypusine synthase and eucaryotic translation initiation factor 5A from tomato. J. Biol. Chem. 2001 May 18;276(20):17541-9
Gene Ontology	biological process peptidyl-lysine modification to hypusine
Pathway	MetaCyc PWY-5905
Sequence	AF296077.1 | mRNA | CDS | Protein
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR11703 2.9E-212 2 369 IPR002773 Deoxyhypusine synthase SUPERFAMILY SSF52467 1.1E-139 10 371 No hit NA TIGRFAM TIGR00321 4.7E-110 30 367 IPR002773 Deoxyhypusine synthase Pfam PF01916 9.4E-130 43 368 IPR002773 Deoxyhypusine synthase