Basic information
Locus name	AT3G04060
Alias	ANAC046
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Transcription regulation:NAC
Effect for Senescence	promote
Gene Description	The NAC transcription factor ANAC046 is a positive regulator of chlorophyll degradation and senescence in Arabidopsis leaves.
Evidence	Genetic evidence [Ref 1]
References	1: Oda-Yamamizo C, Mitsuda N, Sakamoto S, Ogawa D, Ohme-Takagi M, Ohmiya A. The NAC transcription factor ANAC046 is a positive regulator of chlorophyll degradation and senescence in Arabidopsis leaves. Sci Rep. 2016 Mar 29;6:23609. doi: 10.1038/srep23609. Erratum in: Sci Rep. 2016 Oct 19;6:35125.
Gene Ontology	biological process regulation of transcription, DNA-dependent molecular function DNA binding
Sequence	AT3G04060.1 | Genomic | mRNA | CDS | Protein
Mutant information
Mutated 1 Mutant name anac046 Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type T-DNA
Mutated 2 Mutant name ANAC046ox Mutant/Transgenic transgene Ecotype Col-0 Mutagenesis type transgenic
Mutated 3 Mutant name ANAC046SRDX Mutant/Transgenic transgene Ecotype Col-0 Mutagenesis type transgenic
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT3G04060.1  miRNA: ath-miR396b miRNA: ath-miR396b mfe: -23.2 kcal/mol  p-value: 0.049555  position:  248  target 5' G   A          G     U 3'             GAG UC AGGAGGU GUGGA              UUC AG UCUUUCG CACCU   miRNA  3'     A  U       A     U 5'
Ortholog Group
Ortholog Groups: OG5_164511	Accession Taxon NP_187056 ( AT3G04060 ) Arabidopsis thaliana NP_197328 Arabidopsis thaliana NP_974800 Arabidopsis thaliana NP_001049994 Oryza sativa Japonica Group NP_001060671 Oryza sativa Japonica Group 29950.m001171 Ricinus communis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR31744 1.6E-162 1 335 No hit NA SUPERFAMILY SSF101941 3.9E-62 17 172 IPR003441 No apical meristem (NAM) protein Pfam PF02365 4.2E-52 20 146 IPR003441 No apical meristem (NAM) protein ProSiteProfiles PS51005 58.0 20 171 IPR003441 No apical meristem (NAM) protein
Mutant Image
Transgenic plants overexpressing ANAC046 exhibited an early-senescence phenotype and a lower Chl content in comparison with the wild-type plants, whereas loss-of-function mutants exhibited a delayed-senescence phenotype and a higher Chl content.