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| Locus name | AT5G62000 |
| Alias | ARF2/HSS/ORE14 |
| Organism | Arabidopsis thaliana |
| Taxonomic identifier | [NCBI] |
| Function category | Hormone response pathway:Auxin |
| Effect for Senescence | promote |
| Gene Description | Encodes an auxin response factor. Mutants have many defects including enlarged rosette leaves, reduced fertility, later senescence, hypocotyl elongation defects, enlarged seeds and enlarged cotyledons. May not mediate auxin effects. Increase in seed size due to increased cell proliferation. |
| Evidence | Genetic evidence:Mutant [Ref 1, Ref 2]; Genomic evidence:microarray data [Ref 3] |
| References | 1: Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JWAUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana.Development 2005 Oct;132(20):4563-742: Lim PO, Lee IC, Kim J, Kim HJ, Ryu JS, Woo HR, Nam HGAuxin response factor 2 (ARF2) plays a major role in regulating auxin-mediated leaf longevity.J. Exp. Bot. 2010 Mar;61(5):1419-303: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJComparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis.Plant J. 2005 May;42(4):567-85 |
| Gene Ontology | |
| Sequence | AT5G62000.1 | Genomic | mRNA | CDS | Protein
AT5G62000.2 | Genomic | mRNA | CDS | Protein
AT5G62000.3 | Genomic | mRNA | CDS | Protein
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| Mutated 1 | | Mutant name |
arf2-8 | | Mutant/Transgenic |
mutant | | Ecotype |
Col-0 |
| Mutagenesis type |
T-DNA insertion_knock out |
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| Mutated 3 | | Mutant name |
arf1-5 arf2-8 | | Mutant/Transgenic |
mutant | | Ecotype |
Col-0 |
| Mutagenesis type |
T-DNA insertion_knock out |
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| Mutated 4 | | Mutant name |
arf2-5 | | Mutant/Transgenic |
mutant | | Ecotype |
Col-0 |
| Mutagenesis type |
T-DNA insertion_knock out |
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| Mutated 5 | | Mutant name |
arf1-4 dsARF2 | | Mutant/Transgenic |
mutant and transgene | | Ecotype |
Ws |
| Mutagenesis type |
T-DNA insertion_knock out and RNAi |
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| Mutated 6 | | Mutant name |
dsARF2 | | Mutant/Transgenic |
transgenic | | Ecotype |
Ws |
| Mutagenesis type |
RNAi |
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| Sampling |
Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ.
Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis.
Plant J. 2005 May;42(4) |
| Comparation |
Legends:
Col: wild type, indicates the ratio of expression in senescing leaves/green leaves.
NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type.
D/L: ratio of DARK 5d/control.
CD: Cell Death, ratio of starved cell suspension culture/control.
Genes showing at least 3 fold (ratio) up regulation during leaf senescence. |
miRNA Interaction  | |
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| Details | | target: AT5G62000.1
miRNA: ath-miR868
miRNA: ath-miR868
mfe: -22.9 kcal/mol
p-value: 0.054702
position: 3055
target 5' U C C 3'
CAUUGUC AGCGCU GGGAA
GUAAUAG UCGUGA UUCUU
miRNA 3' C A C 5'
target: AT5G62000.2
miRNA: ath-miR868
miRNA: ath-miR868
mfe: -22.9 kcal/mol
p-value: 0.052712
position: 2929
target 5' U C C 3'
CAUUGUC AGCGCU GGGAA
GUAAUAG UCGUGA UUCUU
miRNA 3' C A C 5'
target: AT5G62000.3
miRNA: ath-miR868
miRNA: ath-miR868
mfe: -22.9 kcal/mol
p-value: 0.052788
position: 2929
target 5' U C C 3'
CAUUGUC AGCGCU GGGAA
GUAAUAG UCGUGA UUCUU
miRNA 3' C A C 5'
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| Ortholog Group | |
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| Ortholog Groups: OG5_134025 | |
| Cross Link | |
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| Mutant Image | |
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| arf2-8(CS24602)delayed flowering and senescence | |
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| Localization | nucleus |
| Evidence | SUBAcon |
| Pubmed ID | 23180787 |
