Basic information
Locus name	AT5G45900
Alias	APG7
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Nutrient recycling:Autophagy
Effect for Senescence	delay
Gene Description	Component of autophagy conjugation pathway. Required for proper senescence.
Evidence	Genetic evidence:Mutant [Ref 1]; Genomic evidence:microarray data [Ref 2]
References	1: Doelling JH, Walker JM, Friedman EM, Thompson AR, Vierstra RD The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana. J. Biol. Chem. 2002 Sep 6;277(36):33105-14 2: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85
Gene Ontology	biological process autophagy cellular component cytoplasm molecular function catalytic activity
Sequence	AT5G45900.1 | Genomic | mRNA | CDS | Protein
Mutant information
Mutant name	apg7-1
Mutant/Transgenic	mutant
Ecotype	Ws
Mutagenesis type	T-DNA insertion_knock out
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT5G45900.1  miRNA: ath-miR779 miRNA: ath-miR779 mfe: -22.4 kcal/mol  p-value: 0.034600  position:  139  target 5' C       A             U 3'             GUCGACG AGGUUUCUGG CA              CAGUUGC UUUAAAGGUU GU   miRNA  3' U                  A    5'
Ortholog Group
Ortholog Groups: OG5_128264	Accession Taxon NP_568652 ( AT5G45900 ) Arabidopsis thaliana 114160 Chlamydomonas reinhardtii ACO65444 Micromonas sp. RCC299 NP_001043569 Oryza sativa Japonica Group e_gw1.06.00.89.1 Ostreococcus tauri e_gw1.73.14.1 Physcomitrella patens subsp. patens 29900.m001609 Ricinus communis thaps1_ua_kg.chr_4000167 Thalassiosira pseudonana CCMP1335 XP_002951753 Volvox carteri f. nagariensis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR10953 4.4E-249 9 665 No hit NA TIGRFAM TIGR01381 0.0 13 692 IPR006285 Ubiquitin-like modifier-activating enzyme Atg7 SUPERFAMILY SSF69572 2.5E-44 351 664 IPR009036 Molybdenum cofactor biosynthesis, MoeB Pfam PF00899 1.3E-29 357 508 IPR000594 UBA/THIF-type NAD/FAD binding fold
Mutant Image
atg7 (SALK_057606C) mutant exhibited early senescence phenotype compared with wild type Col-0 (7-week-old).
Subcellular Localization
Localization	cytosol
Evidence	SUBAcon
Pubmed ID	23180787