Basic information
Locus name	AT5G53350
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Protein degradation/modification:protease
Effect for Senescence	Unclear
Gene Description	ATP-dependent Clp protease subunit
Evidence	Molecular evidence: SSH and Northern [Ref 1]; Genomic evidence:microarray data [Ref 2]
References	1: Gepstein S, Sabehi G, Carp MJ, Hajouj T, Nesher MF, Yariv I, Dor C, Bassani M Large-scale identification of leaf senescence-associated genes. Plant J. 2003 Dec;36(5):629-42 2: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85
Gene Ontology	biological process protein folding molecular function ATP binding nucleoside-triphosphatase activity nucleotide binding unfolded protein binding
Sequence	AT5G53350.1 | Genomic | mRNA | CDS | Protein
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
Ortholog Group
Ortholog Groups: OG5_128011	Accession Taxon NP_564423 Arabidopsis thaliana NP_568714 Arabidopsis thaliana NP_568792 ( AT5G53350 ) Arabidopsis thaliana CMT045C Cyanidioschyzon merolae strain 10D 196541 Chlamydomonas reinhardtii ACO69647 Micromonas sp. RCC299 NP_001045031 Oryza sativa Japonica Group NP_001047160 Oryza sativa Japonica Group estExt_gwp_GeneWisePlus.C_Chr_01.00010257 Ostreococcus tauri e_gw1.98.158.1 Physcomitrella patens subsp. patens gw1.163.18.1 Physcomitrella patens subsp. patens gw1.171.20.1 Physcomitrella patens subsp. patens 29609.m000575 Ricinus communis 29827.m002673 Ricinus communis 30024.m001707 Ricinus communis gw1.9.26.1 Thalassiosira pseudonana CCMP1335 XP_002950833 Volvox carteri f. nagariensis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR11262 5.6E-275 7 565 No hit NA TIGRFAM TIGR00382 4.0E-151 155 525 IPR004487 Clp protease, ATP-binding subunit ClpX SUPERFAMILY SSF52540 1.1E-62 156 519 IPR027417 P-loop containing nucleoside triphosphate hydrolase Pfam PF07724 3.2E-44 222 426 IPR013093 ATPase, AAA-2 SMART SM00382 2.2E-11 222 384 IPR003593 AAA+ ATPase domain Pfam PF10431 3.8E-14 433 508 IPR019489 Clp ATPase, C-terminal SMART SM01086 9.0E-24 433 529 IPR019489 Clp ATPase, C-terminal
Subcellular Localization
Localization 1 Localization mitochondria Evidence Localization with GFP-fusion protein (GFP:cDNA) Pubmed ID 10737809
Localization 2 Localization mitochondrion Evidence SUBAcon Pubmed ID 23180787