Basic information
Locus name	AT1G21240
Alias	WAK1
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Lipid/Carbohydrate metabolism
Effect for Senescence	Unclear
Gene Description	Wall-associated kinase 1 (WAK1), putative. Developmental senescence-enhanced genes dependent on SA pathway.
Evidence	Genomice evidence: microarray data [Ref 1]
References	1: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85
Gene Ontology	biological process protein phosphorylation molecular function ATP binding calcium ion binding polysaccharide binding protein kinase activity protein serine/threonine kinase activity
Sequence	AT1G21240.1 | Genomic | mRNA | CDS | Protein
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT1G21240.1  miRNA: ath-miR168a miRNA: ath-miR168a mfe: -30.2 kcal/mol  p-value: 0.020495  position:  2137  target 5' A             A      A 3'             UCUUG UCUGCAC AGGCGA              AGGGC GGACGUG UUCGCU   miRNA  3' A     U       G        5'  target: AT1G21240.1  miRNA: ath-miR168b miRNA: ath-miR168b mfe: -30.2 kcal/mol  p-value: 0.026900  position:  2137  target 5' A             A      A 3'             UCUUG UCUGCAC AGGCGA              AGGGC GGACGUG UUCGCU   miRNA  3' A     U       G        5'  target: AT1G21240.1  miRNA: ath-miR2111b miRNA: ath-miR2111b mfe: -27.6 kcal/mol  p-value: 0.007614  position:  2089  target 5' U   C   U A            G 3'             GGU GGA C GUAUCCUGAGGA              CCA UUU G CAUAGGGCUCCU   miRNA  3'           A            A 5'  target: AT1G21240.1  miRNA: ath-miR413 miRNA: ath-miR413 mfe: -23.1 kcal/mol  p-value: 0.132320  position:  184  target 5' U    C U G           C 3'             GUGU G G AAGAGAAGCUA              CACG C U UUCUCUUUGAU   miRNA  3'      U U G           A 5'
Ortholog Group
Ortholog Groups: OG5_133796	Accession Taxon NP_173544 Arabidopsis thaliana NP_173546 Arabidopsis thaliana NP_173547 ( AT1G21240 ) Arabidopsis thaliana NP_173549 Arabidopsis thaliana NP_189176 Arabidopsis thaliana NP_564137 Arabidopsis thaliana NP_001043036 Oryza sativa Japonica Group NP_001045654 Oryza sativa Japonica Group NP_001047473 Oryza sativa Japonica Group NP_001048454 Oryza sativa Japonica Group NP_001048457 Oryza sativa Japonica Group NP_001048474 Oryza sativa Japonica Group NP_001052352 Oryza sativa Japonica Group NP_001052558 Oryza sativa Japonica Group NP_001052561 Oryza sativa Japonica Group NP_001052564 Oryza sativa Japonica Group NP_001052567 Oryza sativa Japonica Group NP_001052568 Oryza sativa Japonica Group NP_001053322 Oryza sativa Japonica Group NP_001053746 Oryza sativa Japonica Group NP_001053747 Oryza sativa Japonica Group NP_001056769 Oryza sativa Japonica Group NP_001056930 Oryza sativa Japonica Group NP_001063440 Oryza sativa Japonica Group NP_001063441 Oryza sativa Japonica Group NP_001063442 Oryza sativa Japonica Group NP_001063443 Oryza sativa Japonica Group 29648.m001949 Ricinus communis
Cross Link
Database Entry ID E-value Start End InterPro ID Description Pfam PF13947 2.8E-21 29 129 IPR025287 Wall-associated receptor kinase galacturonan-binding domain PANTHER PTHR24420 2.3E-251 239 725 No hit NA ProSiteProfiles PS50026 7.4 245 289 IPR000742 Epidermal growth factor-like domain SMART SM00181 2.3 248 292 IPR000742 Epidermal growth factor-like domain SUPERFAMILY SSF57196 1.2E-5 288 332 No hit NA Pfam PF07645 2.1E-10 293 328 IPR001881 EGF-like calcium-binding ProSitePatterns PS01187 NA 293 319 IPR018097 EGF-like calcium-binding, conserved site ProSiteProfiles PS50026 10.9 293 331 IPR000742 Epidermal growth factor-like domain SMART SM00179 4.8E-7 293 334 IPR001881 EGF-like calcium-binding SMART SM00181 0.3 296 334 IPR000742 Epidermal growth factor-like domain ProSitePatterns PS00010 NA 310 321 IPR000152 EGF-type aspartate/asparagine hydroxylation site SUPERFAMILY SSF56112 2.1E-75 397 686 IPR011009 Protein kinase-like domain ProSiteProfiles PS50011 37.4 415 698 IPR000719 Protein kinase, catalytic domain SMART SM00220 1.7E-30 415 688 IPR002290 Serine/threonine- / dual specificity protein kinase, catalytic domain Pfam PF00069 1.6E-45 416 682 IPR000719 Protein kinase, catalytic domain ProSitePatterns PS00108 NA 536 548 IPR008271 Serine/threonine-protein kinase, active site
Subcellular Localization
Localization 1 Localization plasma membrane Evidence Localization with GFP-fusion protein (GFP:cDNA) Pubmed ID 10737809
Localization 2 Localization plasma membrane Evidence SUBAcon Pubmed ID 23180787