Basic information
GenBank ID	NM_001111966
Alias	KN1
Organism	Maize (Zea mays)
Taxonomic identifier	[NCBI]
Function category	Transcriptional regulation:HB(homeobox)
Effect for Senescence	delay
Gene Description	Kn1 gene encodes a homeodomain-containing protein, the first identified in the plant kingdom. Sequence comparisons strongly suggest that Kn1 acts as a transcription factor. Distal expression of knotted1 in maize leaves leads to reestablishment of proximal/distal patterning and leaf dissection.The maize transcription factor KNOTTED1 directly regulates the gibberellin catabolism gene ga2ox1.The interaction of knotted1 and thick tassel dwarf1 in vegetative and reproductive meristems of maize
Evidence	Genetic evidence:Transgene [Ref 1, Ref 2, Ref 3, Ref 4, Ref 5, Ref 6]
References	1: Ori N, Juarez MT, Jackson D, Yamaguchi J, Banowetz GM, Hake S Leaf senescence is delayed in tobacco plants expressing the maize homeobox gene knotted1 under the control of a senescence-activated promoter. Plant Cell 1999 Jun;11(6):1073-80 2: Vollbrecht E, Veit B, Sinha N, Hake S The developmental gene Knotted-1 is a member of a maize homeobox gene family. Nature 1991 Mar 21;350(6315):241-3 3: Lunde C, Hake S The interaction of knotted1 and thick tassel dwarf1 in vegetative and reproductive meristems of maize. Genetics 2009 Apr;181(4):1693-7 4: Ramirez J, Bolduc N, Lisch D, Hake S Distal expression of knotted1 in maize leaves leads to reestablishment of proximal/distal patterning and leaf dissection. Plant Physiol. 2009 Dec;151(4):1878-88 5: Singh DP, Filardo FF, Storey R, Jermakow AM, Yamaguchi S, Swain SM Overexpression of a gibberellin inactivation gene alters seed development, KNOX gene expression, and plant development in Arabidopsis. Physiol Plant 2010 Jan;138(1):74-90 6: Bolduc N, Hake S The maize transcription factor KNOTTED1 directly regulates the gibberellin catabolism gene ga2ox1. Plant Cell 2009 Jun;21(6):1647-58
Gene Ontology	cellular component nucleus molecular function DNA binding
Sequence	NM_001111966.2 | mRNA | CDS | Protein
Mutant information
Mutant name	SAG:kn1 tobacco plant
Mutant/Transgenic	transgenic
Ecotype	tobacco(Nicotiana tabacum cv Wisconsin 38)
Mutagenesis type	transgene
miRNA Interaction
Details	target: NM_001111966  miRNA: zma-miR162 miRNA: zma-miR162 mfe: -27.9 kcal/mol  p-value: 0.136683  position:  294  target 5' C        C       C    G 3'             GUCG UAG GCCGCUG CGCC              UAGC AUU UGGUGAC GCGG   miRNA  3' C    U                G 5'  target: NM_001111966  miRNA: zma-miR171h miRNA: zma-miR171h mfe: -27.4 kcal/mol  p-value: 0.035749  position:  654  target 5' G              U G    G 3'             ACG GAGCCGGAGC G ACCA              UGU CUCGGCUUUG U UGGU   miRNA  3'     A          U A      5'  target: NM_001111966  miRNA: zma-miR171k miRNA: zma-miR171k mfe: -27.4 kcal/mol  p-value: 0.037369  position:  654  target 5' G              U G    G 3'             ACG GAGCCGGAGC G ACCA              UGU CUCGGCUUUG U UGGU   miRNA  3'     A          U A      5'  target: NM_001111966  miRNA: zma-miR396e miRNA: zma-miR396e mfe: -23.6 kcal/mol  p-value: 0.046739  position:  838  target 5' G   A          A C    G 3'             AGG UCAAGAAGGU G GGAG              UUC AGUUCUUUCG C CCUU   miRNA  3'     A          A A      5'  target: NM_001111966  miRNA: zma-miR396f miRNA: zma-miR396f mfe: -23.6 kcal/mol  p-value: 0.044083  position:  838  target 5' G   A          A C    G 3'             AGG UCAAGAAGGU G GGAG              UUC AGUUCUUUCG C CCUU   miRNA  3'     A          A A      5'  target: NM_001111966  miRNA: zma-miR528a miRNA: zma-miR528a mfe: -31.0 kcal/mol  p-value: 0.039362  position:  608  target 5' A        C C     C      C 3'             GGUGGAGG G GGCAG GCACGG              UUACCUUC U CCGUC CGUGUC   miRNA  3'                         C 5'  target: NM_001111966  miRNA: zma-miR528b miRNA: zma-miR528b mfe: -31.0 kcal/mol  p-value: 0.048232  position:  608  target 5' A        C C     C      C 3'             GGUGGAGG G GGCAG GCACGG              UUACCUUC U CCGUC CGUGUC   miRNA  3'                         C 5'  target: NM_001111966  miRNA: zma-miR162 miRNA: zma-miR162 mfe: -27.9 kcal/mol  p-value: 0.136683  position:  294  target 5' C        C       C    G 3'             GUCG UAG GCCGCUG CGCC              UAGC AUU UGGUGAC GCGG   miRNA  3' C    U                G 5'  target: NM_001111966  miRNA: zma-miR171h miRNA: zma-miR171h mfe: -27.4 kcal/mol  p-value: 0.035749  position:  654  target 5' G              U G    G 3'             ACG GAGCCGGAGC G ACCA              UGU CUCGGCUUUG U UGGU   miRNA  3'     A          U A      5'  target: NM_001111966  miRNA: zma-miR171k miRNA: zma-miR171k mfe: -27.4 kcal/mol  p-value: 0.037369  position:  654  target 5' G              U G    G 3'             ACG GAGCCGGAGC G ACCA              UGU CUCGGCUUUG U UGGU   miRNA  3'     A          U A      5'  target: NM_001111966  miRNA: zma-miR396e miRNA: zma-miR396e mfe: -23.6 kcal/mol  p-value: 0.046739  position:  838  target 5' G   A          A C    G 3'             AGG UCAAGAAGGU G GGAG              UUC AGUUCUUUCG C CCUU   miRNA  3'     A          A A      5'  target: NM_001111966  miRNA: zma-miR396f miRNA: zma-miR396f mfe: -23.6 kcal/mol  p-value: 0.044083  position:  838  target 5' G   A          A C    G 3'             AGG UCAAGAAGGU G GGAG              UUC AGUUCUUUCG C CCUU   miRNA  3'     A          A A      5'  target: NM_001111966  miRNA: zma-miR528a miRNA: zma-miR528a mfe: -31.0 kcal/mol  p-value: 0.039362  position:  608  target 5' A        C C     C      C 3'             GGUGGAGG G GGCAG GCACGG              UUACCUUC U CCGUC CGUGUC   miRNA  3'                         C 5'  target: NM_001111966  miRNA: zma-miR528b miRNA: zma-miR528b mfe: -31.0 kcal/mol  p-value: 0.048232  position:  608  target 5' A        C C     C      C 3'             GGUGGAGG G GGCAG GCACGG              UUACCUUC U CCGUC CGUGUC   miRNA  3'                         C 5'
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR11850 2.7E-51 100 334 No hit NA Pfam PF03790 1.1E-21 102 145 IPR005540 KNOX1 Pfam PF03791 8.5E-23 157 203 IPR005541 KNOX2 Pfam PF03789 1.5E-9 242 263 IPR005539 ELK ProSiteProfiles PS51213 11.3 242 262 IPR005539 ELK ProSiteProfiles PS50071 12.7 262 325 IPR001356 Homeodomain SUPERFAMILY SSF46689 7.7E-20 263 337 IPR009057 Homeodomain-like SMART SM00389 1.6E-12 264 329 IPR001356 Homeodomain Pfam PF05920 1.9E-15 282 321 IPR008422 Homeobox KN domain ProSitePatterns PS00027 NA 300 323 IPR017970 Homeobox, conserved site