Basic information
Locus name	AT1G73500
Alias	MKK9
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Signal transduction:MKK cascade
Effect for Senescence	promote
Gene Description	member of MAP Kinase Kinase family. Autophosphorylates and also phosphorylates MPK3 and MPK6
Evidence	Genetic evidence:Mutant [Ref 1]; Genomic evidence:microarray data [Ref 2]
References	1: Zhou C, Cai Z, Guo Y, Gan S An arabidopsis mitogen-activated protein kinase cascade, MKK9-MPK6, plays a role in leaf senescence. Plant Physiol. 2009 May;150(1):167-77 2: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85
Gene Ontology	biological process protein phosphorylation molecular function ATP binding protein kinase activity protein serine/threonine kinase activity
Sequence	AT1G73500.1 | Genomic | mRNA | CDS | Protein
Mutant information
Mutated 1 Mutant name mkk9-1 Mutant/Transgenic mutant Ecotype Col-0 Mutagenesis type T-DNA insertion_knock out
Mutated 2 Mutant name 35S:MKK9 Mutant/Transgenic transgenic Ecotype Col-0 Mutagenesis type transgene
Mutated 3 Mutant name 35S:MKK9mpk6 Mutant/Transgenic mutant and trangenic(Double mutant) Ecotype Col-0 Mutagenesis type cross
Mutated 4 Mutant name DEXin:MKK9 Mutant/Transgenic transgenic Ecotype Col-0 Mutagenesis type transgene
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. CD: Cell Death, ratio of starved cell suspension culture/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT1G73500.1  miRNA: ath-miR854a miRNA: ath-miR854a mfe: -29.1 kcal/mol  p-value: 0.010936  position:  161  target 5' U                    G 3'             CUUCUUCUCUCUAUUC CAU              GAGGAGGAGGGAUAGG GUA   miRNA  3'                  A   G 5'  target: AT1G73500.1  miRNA: ath-miR854b miRNA: ath-miR854b mfe: -29.1 kcal/mol  p-value: 0.012367  position:  161  target 5' U                    G 3'             CUUCUUCUCUCUAUUC CAU              GAGGAGGAGGGAUAGG GUA   miRNA  3'                  A   G 5'  target: AT1G73500.1  miRNA: ath-miR854c miRNA: ath-miR854c mfe: -29.1 kcal/mol  p-value: 0.010873  position:  161  target 5' U                    G 3'             CUUCUUCUCUCUAUUC CAU              GAGGAGGAGGGAUAGG GUA   miRNA  3'                  A   G 5'  target: AT1G73500.1  miRNA: ath-miR854d miRNA: ath-miR854d mfe: -29.1 kcal/mol  p-value: 0.011557  position:  161  target 5' U                    G 3'             CUUCUUCUCUCUAUUC CAU              GAGGAGGAGGGAUAGG GUA   miRNA  3'                  A   G 5'  target: AT1G73500.1  miRNA: ath-miR854e miRNA: ath-miR854e mfe: -29.1 kcal/mol  p-value: 0.012756  position:  161  target 5' U                    G 3'             CUUCUUCUCUCUAUUC CAU              GAGGAGGAGGGAUAGG GUA   miRNA  3'                  A   G 5'
Ortholog Group
Ortholog Groups: OG5_150257	Accession Taxon NP_173271 Arabidopsis thaliana NP_175577 Arabidopsis thaliana NP_177492 ( AT1G73500 ) Arabidopsis thaliana NP_187274 Arabidopsis thaliana NP_188759 Arabidopsis thaliana NP_001048341 Oryza sativa Japonica Group NP_001057028 Oryza sativa Japonica Group estExt_Genewise1.C_160199 Physcomitrella patens subsp. patens estExt_fgenesh1_pm.C_1140016 Physcomitrella patens subsp. patens 29748.m000383 Ricinus communis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR24361 7.1E-104 1 308 No hit NA ProSiteProfiles PS50011 45.4 47 306 IPR000719 Protein kinase, catalytic domain SMART SM00220 5.5E-83 47 306 IPR002290 Serine/threonine- / dual specificity protein kinase, catalytic domain SUPERFAMILY SSF56112 10.0E-76 47 308 IPR011009 Protein kinase-like domain Pfam PF00069 1.2E-58 48 306 IPR000719 Protein kinase, catalytic domain ProSitePatterns PS00107 NA 53 76 IPR017441 Protein kinase, ATP binding site ProSitePatterns PS00108 NA 163 175 IPR008271 Serine/threonine-protein kinase, active site
Mutant Image
Overexpression of MKK9 (A) induced H2O2 production (D), cell death (C)and early senescence (B).