Basic information
Locus name	AT5G39510
Alias	VTI11
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Nutrient recycling
Effect for Senescence	delay
Gene Description	Encodes a member of SNARE gene family. Homologous with yeast VTI1 and is involved in vesicle transport. Mutant alleles such as sgr4/zig are defective in the shoots response to gravity resulting in a zigzag growth pattern of the stem. Involved in protein trafficking to lytic vacuoles. Can conditionally substitute VTI12 in protein storage vacuole trafficking when plants are devoid of VTI12.Under nutrient-poor conditions, vti12 showed an accelerated senescence phenotype
Evidence	Molecular evidence [Ref 1]
References	1: Hashiguchi Y, Niihama M, Takahashi T, Saito C, Nakano A, Tasaka M, Morita MT Loss-of-function mutations of retromer large subunit genes suppress the phenotype of an Arabidopsis zig mutant that lacks Qb-SNARE VTI11. Plant Cell 2010 Jan;22(1):159-72 2: Niihama M, Takemoto N, Hashiguchi Y, Tasaka M, Morita MT ZIP genes encode proteins involved in membrane trafficking of the TGN-PVC/vacuoles. Plant Cell Physiol. 2009 Dec;50(12):2057-68 3: Limpens E, Ivanov S, van Esse W, Voets G, Fedorova E, Bisseling T Medicago N2-fixing symbiosomes acquire the endocytic identity marker Rab7 but delay the acquisition of vacuolar identity. Plant Cell 2009 Sep;21(9):2811-28 4: Shirakawa M, Ueda H, Shimada T, Nishiyama C, Hara-Nishimura I Vacuolar SNAREs function in the formation of the leaf vascular network by regulating auxin distribution. Plant Cell Physiol. 2009 Jul;50(7):1319-28 5: Ebine K, Okatani Y, Uemura T, Goh T, Shoda K, Niihama M, Morita MT, Spitzer C, Otegui MS, Nakano A, Ueda T A SNARE complex unique to seed plants is required for protein storage vacuole biogenesis and seed development of Arabidopsis thaliana. Plant Cell 2008 Nov;20(11):3006-21 6: Sanmartin M, Ordo_ez A, Sohn EJ, Robert S, Sanchez-Serrano JJ, Surpin MA, Raikhel NV, Rojo E Divergent functions of VTI12 and VTI11 in trafficking to storage and lytic vacuoles in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 2007 Feb 27;104(9):3645-50 7: Song J, Lee MH, Lee GJ, Yoo CM, Hwang I Arabidopsis EPSIN1 plays an important role in vacuolar trafficking of soluble cargo proteins in plant cells via interactions with clathrin, AP-1, VTI11, and VSR1. Plant Cell 2006 Sep;18(9):2258-74 8: Niihama M, Uemura T, Saito C, Nakano A, Sato MH, Tasaka M, Morita MT Conversion of functional specificity in Qb-SNARE VTI1 homologues of Arabidopsis. Curr. Biol. 2005 Mar 29;15(6):555-60 9: Surpin M, Zheng H, Morita MT, Saito C, Avila E, Blakeslee JJ, Bandyopadhyay A, Kovaleva V, Carter D, Murphy A, Tasaka M, Raikhel N The VTI family of SNARE proteins is necessary for plant viability and mediates different protein transport pathways. Plant Cell 2003 Dec;15(12):2885-99 10: Yano D, Sato M, Saito C, Sato MH, Morita MT, Tasaka M A SNARE complex containing SGR3/AtVAM3 and ZIG/VTI11 in gravity-sensing cells is important for Arabidopsis shoot gravitropism. Proc. Natl. Acad. Sci. U.S.A. 2003 Jul 8;100(14):8589-94 11: Sanderfoot AA, Kovaleva V, Bassham DC, Raikhel NV Interactions between syntaxins identify at least five SNARE complexes within the Golgi/prevacuolar system of the Arabidopsis cell. Mol. Biol. Cell 2001 Dec;12(12):3733-43
Gene Ontology	biological process intracellular protein transport vesicle-mediated transport cellular component membrane
Sequence	AT5G39510.1 | Genomic | mRNA | CDS | Protein
Ortholog Group
Ortholog Groups: OG5_127758	Accession Taxon NP_198767 ( AT5G39510 ) Arabidopsis thaliana NP_564255 Arabidopsis thaliana CMJ056C Cyanidioschyzon merolae strain 10D 187998 Chlamydomonas reinhardtii ACO62722 Micromonas sp. RCC299 NP_001043348 Oryza sativa Japonica Group NP_001044021 Oryza sativa Japonica Group 0400010231 Ostreococcus tauri estExt_Genewise1.C_20017 Physcomitrella patens subsp. patens estExt_gwp_gw1.C_4820006 Physcomitrella patens subsp. patens 29635.m000455 Ricinus communis 29813.m001538 Ricinus communis 30138.m004042 Ricinus communis estExt_thaps1_ua_kg.C_chr_40074 Thalassiosira pseudonana CCMP1335 thaps1_ua_kg.chr_23000017 Thalassiosira pseudonana CCMP1335 XP_002949312 Volvox carteri f. nagariensis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR21230 1.2E-139 3 221 No hit NA SUPERFAMILY SSF47661 3.8E-25 6 93 IPR010989 t-SNARE Pfam PF05008 1.8E-24 12 90 IPR007705 Vesicle transport v-SNARE, N-terminal Pfam PF12352 1.1E-24 128 193 No hit NA SUPERFAMILY SSF58038 1.0E-17 130 189 No hit NA
Subcellular Localization
Localization 1 Localization Golgi apparatus Evidence Localization with GFP-fusion protein (GFP:cDNA) Pubmed ID 10737809
Localization 2 Localization vacuole Evidence SUBAcon Pubmed ID 23180787