Leaf Senescence DataBase

Basic information

Locus name

AT5G60360

Alias

ALEU/SAG2

Organism

Arabidopsis thaliana

Taxonomic identifier

[NCBI]

Function category

Nutrient recycling:mobilization of nitrogen

Effect for Senescence

unclear

Gene Description

Encodes a senescence-associated thiol protease.May play a role in proteolysis leading to mobilization of nitrogen during senescence and starvation

Evidence

Genomic evidence:microarray data [Ref 1]; Molecular evidence: SSH and Northern [Ref 2]

References

1: Hensel LL, Grbi_ V, Baumgarten DA, Bleecker AB
Developmental and age-related processes that influence the longevity and senescence of photosynthetic tissues in arabidopsis.
Plant Cell 1993 May;5(5):553-64

2: Gepstein S, Sabehi G, Carp MJ, Hajouj T, Nesher MF, Yariv I, Dor C, Bassani M
Large-scale identification of leaf senescence-associated genes.
Plant J. 2003 Dec;36(5):629-42

Gene Ontology

biological process	proteolysis
molecular function	cysteine-type peptidase activity

Sequence

miRNA Interaction

Details

target: AT5G60360.1
miRNA: ath-miR396a
miRNA: ath-miR396a
mfe: -30.7 kcal/mol
p-value: 0.000566

position:  617
target 5' A  A                U 3'
           AG UCAGGGAGGUUGUGGA
           UC AGUUCUUUCGACACCU
miRNA  3' G  A                U 5'

target: AT5G60360.1
miRNA: ath-miR396b
miRNA: ath-miR396b
mfe: -30.9 kcal/mol
p-value: 0.000792

position:  616
target 5' A   A                U 3'
           AAG UCAGGGAGGUUGUGGA
           UUC AGUUCUUUCGACACCU
miRNA  3'     A                U 5'

target: AT5G60360.2
miRNA: ath-miR396a
miRNA: ath-miR396a
mfe: -30.7 kcal/mol
p-value: 0.000564

position:  617
target 5' A  A                U 3'
           AG UCAGGGAGGUUGUGGA
           UC AGUUCUUUCGACACCU
miRNA  3' G  A                U 5'

target: AT5G60360.2
miRNA: ath-miR396b
miRNA: ath-miR396b
mfe: -30.9 kcal/mol
p-value: 0.000790

position:  616
target 5' A   A                U 3'
           AAG UCAGGGAGGUUGUGGA
           UUC AGUUCUUUCGACACCU
miRNA  3'     A                U 5'

target: AT5G60360.3
miRNA: ath-miR396a
miRNA: ath-miR396a
mfe: -30.7 kcal/mol
p-value: 0.000549

position:  617
target 5' A  A                U 3'
           AG UCAGGGAGGUUGUGGA
           UC AGUUCUUUCGACACCU
miRNA  3' G  A                U 5'

target: AT5G60360.3
miRNA: ath-miR396b
miRNA: ath-miR396b
mfe: -30.9 kcal/mol
p-value: 0.000771

position:  616
target 5' A   A                U 3'
           AAG UCAGGGAGGUUGUGGA
           UUC AGUUCUUUCGACACCU
miRNA  3'     A                U 5'

Ortholog Group

Ortholog Groups: OG5_132819

Accession	Taxon
NP_001030812	Arabidopsis thaliana
NP_001032106	Arabidopsis thaliana
NP_001078774 ( AT5G60360 )	Arabidopsis thaliana
NP_566880	Arabidopsis thaliana
NP_568921	Arabidopsis thaliana
NP_001063289	Oryza sativa Japonica Group
e_gw1.199.86.1	Physcomitrella patens subsp. patens
29739.m003757	Ricinus communis

Cross Link

Database	Entry ID	E-value	Start	End	InterPro ID	Description
PANTHER	PTHR12411	1.7E-194	5	357	IPR013128	Peptidase C1A, papain
SUPERFAMILY	SSF54001	4.9E-112	54	356	No hit	NA
Pfam	PF08246	1.5E-16	59	115	IPR013201	Proteinase inhibitor I29, cathepsin propeptide
SMART	SM00848	7.3E-22	59	115	IPR013201	Proteinase inhibitor I29, cathepsin propeptide
Pfam	PF00112	3.0E-79	141	356	IPR000668	Peptidase C1A, papain C-terminal
SMART	SM00645	2.2E-113	141	356	IPR000668	Peptidase C1A, papain C-terminal
PRINTS	PR00705	2.0E-10	159	174	IPR000668	Peptidase C1A, papain C-terminal
ProSitePatterns	PS00139	NA	159	170	IPR000169	Cysteine peptidase, cysteine active site
ProSitePatterns	PS00639	NA	303	313	IPR025660	Cysteine peptidase, histidine active site
PRINTS	PR00705	2.0E-10	305	315	IPR000668	Peptidase C1A, papain C-terminal
PRINTS	PR00705	2.0E-10	320	326	IPR000668	Peptidase C1A, papain C-terminal
ProSitePatterns	PS00640	NA	320	339	IPR025661	Cysteine peptidase, asparagine active site

Subcellular Localization

Localization 1
	Localization	extracellular
	Evidence	SUBAcon
	Pubmed ID	23180787

Localization 2
	Localization	vacuole
	Evidence	SUBAcon
	Pubmed ID	23180787