Basic information
Locus name	AT3G49160
Organism	Arabidopsis thaliana
Taxonomic identifier	[NCBI]
Function category	Lipid/Carbohydrate metabolism
Effect for Senescence	unclear
Gene Description	pyruvate kinase family protein similar to SP|Q92122 Pyruvate kinase, muscle isozyme (EC 2.7.1.40) (Cytosolic thyroid hormone binding protein) (CTHBP) {Xenopus laevis}; contains Pfam profile PF00224: Pyruvate kinase, barrel domain
Evidence	Genomic evidence:microarray data [Ref 1]
References	1: Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4):567-85
Gene Ontology	biological process glycolysis molecular function magnesium ion binding potassium ion binding pyruvate kinase activity
Pathway	KEGG map00010 (EC 2.7.1.40) map00230 (EC 2.7.1.40) map00620 (EC 2.7.1.40) map00710 (EC 2.7.1.40) MetaCyc PWY-2221 Reactome REACT_474
Sequence	AT3G49160.1 | Genomic | mRNA | CDS | Protein
Microarray
Sampling	Buchanan-Wollaston V, Page T, Harrison E, Breeze E, Lim PO, Nam HG, Lin JF, Wu SH, Swidzinski J, Ishizaki K, Leaver CJ. Comparative transcriptome analysis reveals significant differences in gene expression and signalling pathways between developmental and dark/starvation-induced senescence in Arabidopsis. Plant J. 2005 May;42(4)
Comparation	Legends: Col: wild type, indicates the ratio of expression in senescing leaves/green leaves. NahG, coi1 and ein2: indicates the ratio of expression in senescing leaves of mutant/senescing wild type. D/L: ratio of DARK 5d/control. Genes showing at least 3 fold (ratio) up regulation during leaf senescence.
miRNA Interaction
Details	target: AT3G49160.1  miRNA: ath-miR831 miRNA: ath-miR831 mfe: -24.9 kcal/mol  p-value: 0.033921  position:  105  target 5' U     C   A       C    U 3'             CAAGA GAG GCGAAGA AUUA              GUUCU CUC UGCUUCU UAGU   miRNA  3'       U   A       C      5'  target: AT3G49160.1  miRNA: ath-miR864-5p miRNA: ath-miR864-5p mfe: -22.4 kcal/mol  p-value: 0.075123  position:  1593  target 5' G      U       A      U 3'             UUUG A UCAGUCA GCCUGG              AAAC U AGUUAGU UGGACU   miRNA  3'      U C       A        5'
Ortholog Group
Ortholog Groups: OG5_177873	Accession Taxon NP_190485 ( AT3G49160 ) Arabidopsis thaliana e_gw1.255.8.1 Physcomitrella patens subsp. patens e_gw1.257.11.1 Physcomitrella patens subsp. patens 30169.m006517 Ricinus communis
Cross Link
Database Entry ID E-value Start End InterPro ID Description PANTHER PTHR11817 4.8E-116 113 694 IPR001697 Pyruvate kinase SUPERFAMILY SSF51621 4.2E-63 222 308 IPR015813 Pyruvate/Phosphoenolpyruvate kinase-like domain Pfam PF00224 8.3E-12 234 319 IPR015793 Pyruvate kinase, barrel SUPERFAMILY SSF50800 6.5E-11 433 530 IPR011037 Pyruvate kinase-like, insert domain Pfam PF00224 4.6E-43 466 694 IPR015793 Pyruvate kinase, barrel SUPERFAMILY SSF51621 4.2E-63 531 696 IPR015813 Pyruvate/Phosphoenolpyruvate kinase-like domain