| Accession |
SAMC306450 |
| Sample name |
M3 |
| Title |
adaptive laboratory evolution strain |
| Sample type |
Microbe |
| Organism |
Saccharomyces cerevisiae |
| Description |
Following the novel ALE strategy, an evolved strain M3 was obtained through adaptive evolution with a 5.1-fold increase in carotenoids yield from 3.9 mg/g CDW (cell dry weight) to 19.7 mg/g CDW in the YPD medium. Through whole-genome resequencing and reverse engineering, loss-of-function mutation of PFK1 was revealed as the cause of increased carotenoids yield. Subsequent transcriptome analysis was conducted to reveal the potential mechanisms for improved carotenoids yield, and strengthening of gluconeogenesis and downregulation of cell wall-related genes were observed in M3. |
| Attributes |
|
Strain
|
Saccharomyces cerevisiae
|
|
Isolation source
|
not applicable
|
|
Collection date
|
2020-03-26
|
|
Geographic location
|
china: Guangdong
|
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Altitude
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Biomaterial provider
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Collected by
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Culture collection
|
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Depth
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|
|
Environment biome
|
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Genotype
|
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Host
|
|
|
Host tissue sampled
|
|
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Identified by
|
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|
Lab host
|
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Latitude and longitude
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Mating type
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Passage history
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Sample size
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Serotype
|
|
|
Serovar
|
|
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Specimen voucher
|
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Temperature
|
|
|
| Release date |
2021-01-05 |
| BioProject Accession |
PRJCA004194 |
| Submitter |
Buli Su (66376646@qq.com)
|
| Organization |
Guangdong Academy of Sciences |
| Submission date |
2021-01-05 |
