Accession PRJCA004194
Title Discovery of a new gene target enabling carotenoids improvement of recombinant Saccharomyces cerevisiae using ethanol-induced adaptive laboratory evolution
Relevance Industrial
Data types Raw sequence reads
Organisms Saccharomyces cerevisiae
Description Adaptive laboratory evolution (ALE) is an increasingly popular technique, which allows screening the strains with excellent phenotype through accumulative positive changes. However, little strategy based on ALE has been previously reported about improving carotenoids yield in S. cerevisiae. Herein, a new ethanol-induced ALE was successfully applied to boost carotenoids accumulation in a carotenoid producer BL03-D-4. Following the novel ALE strategy, an evolved strain M3 was obtained through adaptive evolution with a 5.1-fold increase in carotenoids yield from 3.9 mg/g CDW (cell dry weight) to 19.7 mg/g CDW in the YPD medium. Through whole-genome resequencing and reverse engineering, loss-of-function mutation of PFK1 was revealed as the cause of increased carotenoids yield. Subsequent transcriptome analysis was conducted to reveal the potential mechanisms for improved carotenoids yield, and strengthening of gluconeogenesis and downregulation of cell wall-related genes were observed in M3. This study demonstrates that ethanol is a powerful stress to obtain high carotenoids accumulation strains and provides PFK1 as a new target for improving carotenoids yield in S. cerevisiae.
Sample scope Monoisolate
Release date 2021-01-05
Grants
Agency program Grant ID Grant title
ey-Area Research and Development Program of Guangdong Province 2018B020206001
Submitter Buli    Su  (66376646@qq.com)
Organization Guangdong Academy of Sciences
Submission date 2021-01-05

Project Data

Resource name Description
BioSample (2) -
SAMC306449 wild type
SAMC306450 adaptive laboratory evolution strain
GSA (2) -
CRA003718 RNA-seq of BL03-D-4 and M3
CRA003704 Discovery of a new gene target enabling carotenoids improvement of recombinant Saccharomyces cerevisiae using ethanol-induced adaptive laboratory evolution