Introduction

PCR amplification of DNA is a key preliminary step in many applications of high-throughput sequencing technologies, yet design of novel barcoded primers and taxonomic analysis of novel or existing primers remains a challenging task.PrimerProspector is an open-source software package that allows researchers to develop new primers from collections of sequences and to evaluate existing primers in the context of taxonomic data.PrimerProspector is open-source software available at http://pprospector.sourceforge.netrob.knight@colorado.eduSupplementary data are available at Bioinformatics online.

Publications

  1. PrimerProspector: de novo design and taxonomic analysis of barcoded polymerase chain reaction primers.
    Cite this
    Walters WA, Caporaso JG, Lauber CL, Berg-Lyons D, Fierer N, Knight R, 2011-04-01 - Bioinformatics (Oxford, England)

Credits

  1. William A Walters
    Developer

    Department of Molecular, Cellular, United States of America

  2. J Gregory Caporaso
    Developer

  3. Christian L Lauber
    Developer

  4. Donna Berg-Lyons
    Developer

  5. Noah Fierer
    Developer

  6. Rob Knight
    Investigator

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Summary
AccessionBT000073
Tool TypeApplication
Category
PlatformsLinux/Unix
Technologies
User InterfaceTerminal Command Line
Download Count0
Submitted ByRob Knight