Gene Expression Nebulas
A data portal of transcriptomic profiles analyzed by a unified pipeline across multiple species

Gene Expression Nebulas

A data portal of transcriptome profiles across multiple species

PRJNA513348: The study of the role of POU3F2 in response to UVB treatment

Source: NCBI / GSE124761
Submission Date: Jan 07 2019
Release Date: Jan 08 2019
Update Date: Jan 09 2019

Summary: siRNA KD of POU3F2 directly effect DNA-damage repair and apoptosis genes

Overall Design: 501mel melanoma cell line was treated with either siCTR or siPOU3F2 prior to UVB treatment. The samples were collected at the indicated time

GEN Datasets:
GEND000027
Strategy:
Species:
Tissue:
Healthy Condition:
Cell Line:
Protocol
Growth Protocol: All cell lines were grown at 37°C with 10% CO2 in RPMI-1640 (Gibco BRL, Invitrogen) supplemented with penicillin and streptomycin, 10% fetal bovine serum (FBS, Biosera).
Treatment Protocol: 501mel melanoma cell line was treated with either siCTR prior to UVB treatment; 501mel melanoma cell line was treated with either siPOU3F2 prior to UVB treatment
Extract Protocol: RNA-samples were extracted using QIAGEN RNeasy kit
Library Construction Protocol: Library prep using QuantSeq Forward kit (LEXOGEN #015.96), using 500 ng starting material
Sequencing
Molecule Type: poly(A)+ RNA
Library Source:
Library Layout: SINGLE
Library Strand: Reverse
Platform: ILLUMINA
Instrument Model: Illumina HiSeq 4000
Strand-Specific: Specific
Samples
Basic Information:
Sample Characteristic:
Biological Condition:
Experimental Variables:
Protocol:
Sequencing:
Assessing Quality:
Analysis:
Data Resource GEN Sample ID GEN Dataset ID Project ID BioProject ID Sample ID Sample Name BioSample ID Sample Accession Experiment Accession Release Date Submission Date Update Date Species Race Ethnicity Age Age Unit Gender Source Name Tissue Cell Type Cell Subtype Cell Line Disease Disease State Development Stage Mutation Phenotype Case Detail Control Detail Growth Protocol Treatment Protocol Extract Protocol Library Construction Protocol Molecule Type Library Layout Strand-Specific Library Strand Spike-In Strategy Platform Instrument Model Cell Number Reads Number Gbases AvgSpotLen1 AvgSpotLen2 Uniq Mapping Rate Multiple Mapping Rate Coverage Rate
Publications
BRN2 suppresses apoptosis, reprograms DNA damage repair, and is associated with a high somatic mutation burden in melanoma.
Genes & development . 2019-02-25 [PMID: 30804224]