Gene Expression
Nebulas
Gene Expression NebulasSummary: Sperm cells of seed plants have lost their motility and are transported by the vegetative pollen tube cell for fertilization. The extent to which sperm cells regulate their own transportation is a long-standing debate. By using the novel Arabidopsis double mutant drop1 drop2, we demonstrate here that sperm cells are only passive cargo and that the vegetative tube cell as a vehicle controls the entire journey.
Overall Design: The semi-in-vivo pollen tubes of wild type (WT) and drop mutant using single-cell RNA sequencing
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| Growth Protocol: | pollen tubes were growed on the solid pollen germination medium (SPGM), which10% sucrose, 0.01% boric acid, 5 mM CaCl2, 5 mM KCl, 1 mM MgSO4 and pH=7.5. |
| Treatment Protocol: | About 500-600 double mutant pollen grains were hand-picked under a stereoscopic microscope and pollinated on a single stigma (emasculated for 36 hours) and cut together with the style. When the SIV PTs grew about 300-400 μm outside of the style, they were cut from the end of the style with tiny scissors (VANNAS, 8.5 cm, STR) under a stereo microscope. |
| Extract Protocol: | Poly(A)+ RNA |
| Library Construction Protocol: | The lysed solution was used to generate the cDNA libraries. The cDNA libraries follow the Smart-seq2 protocol. |
| Molecule Type: | poly(A)+ RNA |
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| Library Layout: | PAIRED |
| Library Strand: | - |
| Platform: | ILLUMINA |
| Instrument Model: | Illumina HiSeq 4000 |
| Strand-Specific: | Unspecific |
| Data Resource | GEN Sample ID | GEN Dataset ID | Project ID | BioProject ID | Sample ID | Sample Name | BioSample ID | Sample Accession | Experiment Accession | Release Date | Submission Date | Update Date | Species | Race | Ethnicity | Age | Age Unit | Gender | Source Name | Tissue | Cell Type | Cell Subtype | Cell Line | Disease | Disease State | Development Stage | Mutation | Phenotype | Case Detail | Control Detail | Growth Protocol | Treatment Protocol | Extract Protocol | Library Construction Protocol | Molecule Type | Library Layout | Strand-Specific | Library Strand | Spike-In | Strategy | Platform | Instrument Model | Cell Number | Reads Number | Gbases | AvgSpotLen1 | AvgSpotLen2 | Uniq Mapping Rate | Multiple Mapping Rate | Coverage Rate |
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