Growth Protocol:	Cells were resuspended in DMEM/F12 with addition of B27 supplement (1:1000), N2 supplement (1:100), 20 ng/ml FGF-2, 20 ng/ml EGF, 1.6 g/l glucose, 20 ?g/ml insulin and 5 ng/ml BDNF. 10 ?M rock inhibitor (Y-27632, Stemgent 04-0012) was added to increase cell viability. The cells were then plated into dishes coated with poly-L-ornithine (0.01%, Sigma P4957), laminin (5 ?g/ml, Invitrogen 23017-015) and fibronectin (1μg/ml, Corning, 354008,). Medium was changed 12 hr after plating and the cells were passaged using 0.25% trypsin within 3 d and replated to obtain a monolayer of cells at a density of 4–5 × 104 cells/cm2. Half of the media was changed every 2–3 d to allow culture conditioning."
Treatment Protocol:	-
Extract Protocol:	RNA was extracted after single cell capture using C1 single cell auto prep system, with manufacturer protocol.
Library Construction Protocol:	RNA libraries were prepared for sequencing using with Illumina NEXTERA XT kit

Molecule Type:	poly(A)+ RNA
Library Source:
Library Layout:	SINGLE
Library Strand:	-
Platform:	ILLUMINA
Instrument Model:	Illumina HiSeq 2000
Strand-Specific:	Unspecific

Data Resource	GEN Sample ID	GEN Dataset ID	Project ID	BioProject ID	Sample ID	Sample Name	BioSample ID	Sample Accession	Experiment Accession	Release Date	Submission Date	Update Date	Species	Race	Ethnicity	Age	Age Unit	Gender	Source Name	Tissue	Cell Type	Cell Subtype	Cell Line	Disease	Disease State	Development Stage	Mutation	Phenotype	Case Detail	Control Detail	Growth Protocol	Treatment Protocol	Extract Protocol	Library Construction Protocol	Molecule Type	Library Layout	Strand-Specific	Library Strand	Spike-In	Strategy	Platform	Instrument Model	Cell Number	Reads Number	Gbases	AvgSpotLen1	AvgSpotLen2	Uniq Mapping Rate	Multiple Mapping Rate	Coverage Rate