Growth Protocol:	Neuronal differentiation of iPSCs: iPSC colonies were dissociated and plated on AggrewellTM 800 plates (STEMCELL Technologies) in DMEM/F12-GLUTAMAX medium containing 4% B27 supplement without vitamin A (Invitrogen), 1% N2 supplement (Invitrogen), and 55 uM 2-mercaptoethanol, 5μM Y-27632 and 200ng/ml recombinant mouse Noggin (R&D Systems). After 2 days, embryoid bodies (EBs) were collected and plated onto 10-cm bacterial Petri dishes in neuronal medium as above. After two days (day 4), free-floating EBs were collected and plated in neuronal medium, supplemented with only Noggin, onto tissue culture dishes coated with growth factor-reduced Matrigel (BD Bioscience) to allow neural rosette formation. The neuronal medium was changed the next day and supplemented with 20 ng/ml FGF2, 200 ng/ml Noggin, and 200 ng/ml rhDkk1 (R&D Systems). After two or three days, the neural rosettes were manually dissected and replated as free-floating aggregates in 10-cm bacterial Petri dishes in neuronal medium supplemented with FGF2 (10ng/ml) and EGF (10ng/ml). After five days in suspension, free-floating aggregates were plated onto ultra low-attachment 96-well plates (Corning) in neuronal medium supplemented with FGF2 and EGF. Terminal differentiation was started the next day, using a NEUROBASAL-type medium supplemented with 1% N2, 2% B27, 15 mM HEPES, 1X Glutamax, 1X nonessential amino acids (NEAA) and 55 uM 2-mercaptoethanol, supplemented with 200 nM ascorbic acid, 10 ng/ml BDNF (R&D), 10 ng/ml GDNF (R&D) and 1 mM dibutyryl-cAMP (Sigma). Half of the medium was changed twice a week.
Treatment Protocol:	-
Extract Protocol:	Total RNA was extracted using the PicoPure kit (Life Technologies catalog # KIT0204).
Library Construction Protocol:	RNA sequencing was performed using 50ng of total RNA with the SMARTer® Ultra Low RNA kit for Illumina® Sequencing according to the manufacturer's instructions

Molecule Type:	poly(A)+ RNA
Library Source:
Library Layout:	PAIRED
Library Strand:	Forward
Platform:	ILLUMINA
Instrument Model:	Illumina HiSeq 2000
Strand-Specific:	Specific

Data Resource	GEN Sample ID	GEN Dataset ID	Project ID	BioProject ID	Sample ID	Sample Name	BioSample ID	Sample Accession	Experiment Accession	Release Date	Submission Date	Update Date	Species	Race	Ethnicity	Age	Age Unit	Gender	Source Name	Tissue	Cell Type	Cell Subtype	Cell Line	Disease	Disease State	Development Stage	Mutation	Phenotype	Case Detail	Control Detail	Growth Protocol	Treatment Protocol	Extract Protocol	Library Construction Protocol	Molecule Type	Library Layout	Strand-Specific	Library Strand	Spike-In	Strategy	Platform	Instrument Model	Cell Number	Reads Number	Gbases	AvgSpotLen1	AvgSpotLen2	Uniq Mapping Rate	Multiple Mapping Rate	Coverage Rate