URL: | http://primerdepot.nci.nih.gov/ |
Full name: | qPrimerDepot |
Description: | qPrimerDepot is a primer database for quantitative real time PCR |
Year founded: | 2007 |
Last update: | 2007-06-25 |
Version: | v1.0 |
Accessibility: | |
Country/Region: | United States |
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University/Institution: | National Cancer Institute |
Address: | MD 20892-4605 |
City: | Bethesda |
Province/State: | MD |
Country/Region: | United States |
Contact name (PI/Team): | Kevin Gardner |
Contact email (PI/Helpdesk): | gardnerk@mail.nih.gov |
qPrimerDepot: a primer database for quantitative real time PCR. [PMID: 17068075]
Gene expression studies employing high throughput real time PCR methods require finding uniform conditions for optimal amplification of multiple targets, often a daunting task. We developed a primer database, qPrimerDepot, which provides optimized primers for all human and mouse RefSeq genes. These primers are designed to amplify desired templates under unified annealing temperature. For most intron-bearing genes, primers flank one of the largest introns thus minimizing background noise due to genomic DNA contamination. The qPrimerDepot database can be accessed at http://primerdepot.nci.nih.gov/ and http://mouseprimerdepot.nci.nih.gov/. |