SLG1 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: SLG1
Synonym: SLOW GROWTH 1
Description: Encodes a pentatricopeptide repeat protein involved in mitochondrial RNA editing, plant development, and responses to abiotic stresses in Arabidopsis.
Protein Family: PPR
Subclass: E+
Construct Structure: PLS-E-E+
Gene ID & Species: AT5G08490 (Arabidopsis thaliana)
Edited Gene(s): nad3
Editing Type(s): C-to-U (6)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] Functional disruption of the pentatricopeptide protein SLG1 affects mitochondrial RNA editing, plant development, and responses to abiotic stresses in Arabidopsis, The Plant Journal, 2012. [PMID=22248025]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT5G08490 Mitochondrion nad3 250 CDS C-to-U CCU=>UCU
NA=>NA
P=>S
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentSeedling8 daysDirect Sequencing of PCR Products72.00%HighNone22248025
Col-0slg1T-DNA InsertionThe T-DNA is inserted into the gene At5g08490 at 170 bp after the initial codon ATGKnockoutSlow growth and delayed development, as well as altered responses to various abiotic stresses.Seedling8 daysDirect Sequencing of PCR Products0.00%UneditedAbsent22248025
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq84.78%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract86.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract23.00%LowDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract24.00%LowDecreased28761003
Last update: Jul 2021 (version 1.0)