Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Physcomitrella patens subsp. Patens | Δ56-10 | The Nptii Gene Cassette | Restriction sites - Ecorv | Knockout | Slightly reduced growth of the protonemata | Protonema | 3 days | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 20837503 |
| Physcomitrella patens subsp. Patens | Δ56-22 | The Nptii Gene Cassette | Restriction sites - Ecorv | Knockout | Slightly reduced growth of the protonemata | Protonema | 3 days | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 20837503 |
| Physcomitrella patens subsp. Patens | WT | No treatment | No treatment | No treatment | No treatment | Protonema | 3 days | Direct Sequencing of PCR Products | 100.00% | Complete | None | 20837503 |
| NA | 56comp #1 | Complemented with 56comp #1 | Introduced the wild-type PpPPR_56 into PpPPR_56 KO moss line (Δ56–10). A 3× HA epitope tag sequence was inserted in front of the first PPR motif to not affect the editing function of the C-terminal DY | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 97.70%±3.2 | High | Restored | 32348368 |
| NA | 56comp #6 | Complemented with 56comp #6 | Introduced the wild-type PpPPR_56 into PpPPR_56 KO moss line (Δ56–10). A 3× HA epitope tag sequence was inserted in front of the first PPR motif to not affect the editing function of the C-terminal DY | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 93.80%±0.9 | High | Restored | 32348368 |
| NA | 56nad3L variant #2 | Complemented with 56nad3L variant #2 | Introduced 56nad3L variant of the wild-type PpPPR_56 into PpPPR_56 KO moss line (Δ56–10). A 3× HA epitope tag sequence was inserted in front of the first PPR motif to not affect the editing function | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 2.80%±0.60 | Poor | NA | 32348368 |
| NA | 56nad3L variant #9 | Complemented with 56nad3L variant #9 | Introduced 56nad3L variant of the wild-type PpPPR_56 into PpPPR_56 KO moss line (Δ56–10). A 3× HA epitope tag sequence was inserted in front of the first PPR motif to not affect the editing function | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 4.10%±0.50 | Poor | NA | 32348368 |
| NA | 10L[ND] #2 | Complemented with 10L[ND] #2 | PpPPR_56 KO mosses complemented with variant 10L[ND] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 25.00% | Low | Decreased | 32348368 |
| NA | 10L[ND] #4 | Complemented with 10L[ND] #4 | PpPPR_56 KO mosses complemented with variant 10L[ND] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 80.00% | High | Decreased | 32348368 |
| NA | 10L[LS] #3 | Complemented with 10L[LS] #3 | PpPPR_56 KO mosses complemented with variant 10L[LS] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 95.00% | High | Restored | 32348368 |
| NA | 13L[ND] #1 | Complemented with 13L[ND] #1 | PpPPR_56 KO mosses complemented with variant 13L[ND] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 60.00% | High | Decreased | 32348368 |
| NA | 13L[ND] #2 | Complemented with 13L[ND] #2 | PpPPR_56 KO mosses complemented with variant 13L[ND] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 25.00% | Low | Decreased | 32348368 |
| NA | 13L[VS] #1 | Complemented with 13L[VS] #1 | PpPPR_56 KO mosses complemented with variant 13L[VS] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 100.00% | Complete | Restored | 32348368 |
| NA | 1L[NN] #2 | Complemented with 1L[NN] #2 | PpPPR_56 KO mosses complemented with variant 1L[NN] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 44.00% | Medium | Decreased | 32348368 |
| NA | 1L[NN] #3 | Complemented with 1L[NN] #3 | PpPPR_56 KO mosses complemented with variant 1L[NN] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 65.00% | High | Decreased | 32348368 |
| NA | 4L[TD] #1 | Complemented with 4L[TD] #1 | PpPPR_56 KO mosses complemented with variant 4L[TD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 85.00% | High | NA | 32348368 |
| NA | 4L[TD] #3 | Complemented with 4L[TD] #3 | PpPPR_56 KO mosses complemented with variant 4L[TD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 85.00% | High | NA | 32348368 |
| NA | 7L[TN] #1 | Complemented with 7L[TN] #1 | PpPPR_56 KO mosses complemented with variant 7L[TN] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 88.00% | High | NA | 32348368 |
| NA | 7L[TN] #2 | Complemented with 7L[TN] #2 | PpPPR_56 KO mosses complemented with variant 7L[TN] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 76.00% | High | Decreased | 32348368 |
| NA | 10L[NS] #4 | Complemented with 10L[NS] #4 | PpPPR_56 KO mosses complemented with variant 10L[NS] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 9.00% | Poor | Decreased | 32348368 |
| NA | 10L[NS] #8 | Complemented with 10L[NS] #8 | PpPPR_56 KO mosses complemented with variant 10L[NS] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 20.00% | Low | Decreased | 32348368 |
| NA | 13L[NS] #13 | Complemented with 13L[NS] #13 | PpPPR_56 KO mosses complemented with variant 13L[NS] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 65.00% | High | Decreased | 32348368 |
| NA | 13L[NS] #30 | Complemented with 13L[NS] #30 | PpPPR_56 KO mosses complemented with variant 13L[NS] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 56.00% | Medium | Decreased | 32348368 |
| NA | 1L4L7L #1 | Complemented with 1L4L7L #1 | PpPPR_56 KO mosses complemented with variant 1L4L7L | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 72.00% | High | Decreased | 32348368 |
| NA | 1L4L7L #2 | Complemented with 1L4L7L #2 | PpPPR_56 KO mosses complemented with variant 1L4L7L | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 50.00% | Medium | Decreased | 32348368 |
| NA | 10L13L #31 | Complemented with 10L13L #31 | PpPPR_56 KO mosses complemented with variant 10L13L | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 5.00% | Poor | Decreased | 32348368 |
| NA | 10L13L #47 | Complemented with 10L13L #47 | PpPPR_56 KO mosses complemented with variant 10L13L | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 4.00% | Poor | Decreased | 32348368 |
| NA | 56comp 10[LD] #1 | Complemented with 56comp 10[LD] #1 | PpPPR_56 KO mosses complemented with 56comp 10[LD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 98.00% | High | Restored | 32348368 |
| NA | 10L[VD] #5 | Complemented with 10L[VD] #5 | PpPPR_56 KO mosses complemented with 10L[VD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 98.00% | High | Restored | 32348368 |
| NA | 10L[VD] #8 | Complemented with 10L[VD] #8 | PpPPR_56 KO mosses complemented with 10L[VD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 91.00% | High | Restored | 32348368 |
| NA | 10L[MD] #1 | Complemented with 10L[MD] #1 | PpPPR_56 KO mosses complemented with 10L[MD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 95.00% | High | Restored | 32348368 |
| NA | 10L[MD] #7 | Complemented with 10L[MD] #7 | PpPPR_56 KO mosses complemented with 10L[MD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 94.00% | High | Restored | 32348368 |
| NA | 10L[PD] #1 | Complemented with 10L[PD] #1 | PpPPR_56 KO mosses complemented with 10L[PD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 58.00% | Medium | Decreased | 32348368 |
| NA | 10L[PD] #3 | Complemented with 10L[PD] #3 | PpPPR_56 KO mosses complemented with 10L[PD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 46.00% | Medium | Decreased | 32348368 |
| NA | 10L[SD] #2 | Complemented with 10L[SD] #2 | PpPPR_56 KO mosses complemented with 10L[SD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 56.00% | Medium | Decreased | 32348368 |
| NA | 10L[SD] #3 | Complemented with 10L[SD] #3 | PpPPR_56 KO mosses complemented with 10L[SD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 70.00% | High | Decreased | 32348368 |
| NA | 10L[TD] #7 | Complemented with 10L[TD] #7 | PpPPR_56 KO mosses complemented with 10L[TD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 88.00% | High | NA | 32348368 |
| NA | 10L[TD] #8 | Complemented with 10L[TD] #8 | PpPPR_56 KO mosses complemented with 10L[TD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 86.00% | High | NA | 32348368 |
| NA | 10L[QD] #3 | Complemented with 10L[QD] #3 | PpPPR_56 KO mosses complemented with 10L[QD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 93.00% | High | Restored | 32348368 |
| NA | 10L[QD] #24 | Complemented with 10L[QD] #24 | PpPPR_56 KO mosses complemented with 10L[QD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 83.00% | High | NA | 32348368 |
| NA | 10L[KD] #1 | Complemented with 10L[KD] #1 | PpPPR_56 KO mosses complemented with 10L[KD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 55.00% | Medium | Decreased | 32348368 |
| NA | 10L[KD] #2 | Complemented with 10L[KD] #2 | PpPPR_56 KO mosses complemented with 10L[KD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 70.00% | High | Decreased | 32348368 |
| NA | 10L[ED] #4 | Complemented with 10L[ED] #4 | PpPPR_56 KO mosses complemented with 10L[ED] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 8.00% | Poor | Decreased | 32348368 |
| NA | 10L[ED] #7 | Complemented with 10L[ED] #7 | PpPPR_56 KO mosses complemented with 10L[ED] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 4.00% | Poor | Decreased | 32348368 |
| NA | 56comp 13[VD] #1 | Complemented with 56comp 13[VD] #1 | PpPPR_56 KO mosses complemented with 56comp 13[VD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 98.00% | High | Restored | 32348368 |
| NA | 13L[LD] #1 | Complemented with 13L[LD] #1 | PpPPR_56 KO mosses complemented with 13L[LD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 98.00% | High | Restored | 32348368 |
| NA | 13L[LD] #2 | Complemented with 13L[LD] #2 | PpPPR_56 KO mosses complemented with 13L[LD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 88.00% | High | NA | 32348368 |
| NA | 13L[MD] #2 | Complemented with 13L[MD] #2 | PpPPR_56 KO mosses complemented with 13L[MD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 80.00% | High | Decreased | 32348368 |
| NA | 13L[MD] #15 | Complemented with 13L[MD] #15 | PpPPR_56 KO mosses complemented with 13L[MD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 68.00% | High | Decreased | 32348368 |
| NA | 13L[PD] #8 | Complemented with 13L[PD] #8 | PpPPR_56 KO mosses complemented with 13L[PD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 3.00% | Poor | Decreased | 32348368 |
| NA | 13L[PD] #9 | Complemented with 13L[PD] #9 | PpPPR_56 KO mosses complemented with 13L[PD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 3.00% | Poor | Decreased | 32348368 |
| NA | 13L[SD] #1 | Complemented with 13L[SD] #1 | PpPPR_56 KO mosses complemented with 13L[SD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 3.00% | Poor | Decreased | 32348368 |
| NA | 13L[SD] #2 | Complemented with 13L[SD] #2 | PpPPR_56 KO mosses complemented with 13L[SD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 3.00% | Poor | Decreased | 32348368 |
| NA | 13L[TD] #2 | Complemented with 13L[TD] #2 | PpPPR_56 KO mosses complemented with 13L[TD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 88.00% | High | NA | 32348368 |
| NA | 13L[TD] #3 | Complemented with 13L[TD] #3 | PpPPR_56 KO mosses complemented with 13L[TD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 95.00% | High | Restored | 32348368 |
| NA | 13L[QD] #1 | Complemented with 13L[QD] #1 | PpPPR_56 KO mosses complemented with 13L[QD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 13.00% | Poor | Decreased | 32348368 |
| NA | 13L[QD] #6 | Complemented with 13L[QD] #6 | PpPPR_56 KO mosses complemented with 13L[QD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 18.00% | Poor | Decreased | 32348368 |
| NA | 13L[KD] #2 | Complemented with 13L[KD] #2 | PpPPR_56 KO mosses complemented with 13L[KD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 75.00% | High | Decreased | 32348368 |
| NA | 13L[KD] #3 | Complemented with 13L[KD] #3 | PpPPR_56 KO mosses complemented with 13L[KD] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 60.00% | High | Decreased | 32348368 |
| NA | 13L[ED] #1 | Complemented with 13L[ED] #1 | PpPPR_56 KO mosses complemented with 13L[ED] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 3.00% | Poor | Decreased | 32348368 |
| NA | 13L[ED] #2 | Complemented with 13L[ED] #2 | PpPPR_56 KO mosses complemented with 13L[ED] | Knockout | NA | Protonemata | 4-day-old | RT-PCR, cDNA sequencing and chromatographs were analyzed usi | 4.00% | Poor | Decreased | 32348368 |