PPR2263 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: PPR2263
Synonym: Pentatricopeptide Repeat Protein2263
Description: Encodes a DYW-subgroup pentatricopeptide repeat protein required for mitochondrial nad5 and cob transcript editing, mitochondrion biogenesis, and maize growth.
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AC215198.3_FG002 (Zea mays subsp. mays)
Edited Gene(s): nad5    cob
Editing Type(s): C-to-U (4)
Publication(s): [1] PPR2263, a DYW-Subgroup Pentatricopeptide Repeat Protein, Is Required for Mitochondrial nad5 and cob Transcript Editing, Mitochondrion Biogenesis, and Maize Growth, The Plant Cell, 2012. [PMID=22319053]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Zea mays subsp. mays AC215198.3_FG002 Mitochondrion cob 908 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Mutator-induced Biogemma mutant collectionppr2263Mutator insertionMutator insertion leads to a truncated protein lacking three of its 13 PPR repeats as well as the C-terminal E/E+ and DYW domainsHomozygousSmall kernels and developmental delayLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22319053
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products100.00%CompleteNone22319053
Zea mays subsp. mays AC215198.3_FG002 Mitochondrion nad5 1550 CDS C-to-U ACA=>AUA T=>I Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Mutator-induced Biogemma mutant collectionppr2263Mutator insertionMutator insertion leads to a truncated protein lacking three of its 13 PPR repeats as well as the C-terminal E/E+ and DYW domainsHomozygousSmall kernels and developmental delayLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22319053
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products100.00%CompleteNone22319053
Last update: Jul 2021 (version 1.0)