Plant Editosome Database
a curated database of RNA editosome in plants

MEF35 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: MEF35
Synonym: MITOCHONDRIAL EDITING FACTOR 35
Description: Encodes a DYW subgroup PPR protein targeting RNA editing sites in the mitochondrial rpl16, nad4 and cob mRNAs in Arabidopsis thaliana.
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT4G14050 (Arabidopsis thaliana)
Edited Gene(s): rpl16    nad4    cob
Editing Type(s): C-to-U (24)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] The DYW Subgroup PPR Protein MEF35 Targets RNA Editing Sites in the Mitochondrial rpl16, nad4 and cob mRNAs in Arabidopsis thaliana, PLOS One, 2015. [PMID=26470017]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT4G14050 Mitochondrion cob 286 CDS C-to-U CUU=>UUU
NA=>NA		 L=>F
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0No treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products100.00%CompleteNone26470017
SAIL_101_B01mef35-1T-DNA InsertionT-DNA is inserted in the upstreamHomozygousThe plants of mutant line mef35-1 show developmental and adult phenotypes indistinguishable from the WTNANADirect Sequencing of PCR Products0.00%UneditedAbsent26470017
SALK_060464mef35-2T-DNA InsertionT-DNA is inserted in the reading frame encoding the DYW domainHomozygousSeedlings of mutant line mef35-2 initially develop a little slower in comparison to the WT col plantlets, but look similar at the flowering stageNANADirect Sequencing of PCR Products0.00%UneditedAbsent26470017
SALK_060464mef35-2+MEF35ComplementationThe MEF35 NT (wt) Col reading frame is transformed under control of the 35S promoter in vector Pmdc123HomozygousRetarded growthNANADirect Sequencing of PCR ProductsNAHighRestored26470017
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq98.76%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Arabidopsis thaliana AT4G14050 Mitochondrion nad4 1373 CDS C-to-U UCC=>UUC
NA=>NA		 S=>F
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0No treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products100.00%CompleteNone26470017
SAIL_101_B01mef35-1T-DNA InsertionT-DNA is inserted in the upstreamHomozygousThe plants of mutant line mef35-1 show developmental and adult phenotypes indistinguishable from the WTNANADirect Sequencing of PCR Products0.00%UneditedAbsent26470017
SALK_060464mef35-2T-DNA InsertionT-DNA is inserted in the reading frame encoding the DYW domainHomozygousSeedlings of mutant line mef35-2 initially develop a little slower in comparison to the WT col plantlets, but look similar at the flowering stageNANADirect Sequencing of PCR Products0.00%UneditedAbsent26470017
SALK_060464mef35-2+MEF35ComplementationThe MEF35 NT (wt) Col reading frame is transformed under control of the 35S promoter in vector Pmdc123HomozygousRetarded growthNANADirect Sequencing of PCR ProductsNAHighRestored26470017
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq99.11%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract95.00%HighSimilar28761003
Arabidopsis thaliana AT4G14050 Mitochondrion rpl16 209 CDS C-to-U ACU=>AUU
NA=>NA		 T=>I
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0No treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products100.00%CompleteNone26470017
SAIL_101_B01mef35-1T-DNA InsertionT-DNA is inserted in the upstreamHomozygousThe plants of mutant line mef35-1 show developmental and adult phenotypes indistinguishable from the WTNANADirect Sequencing of PCR Products0.00%UneditedAbsent26470017
SALK_060464mef35-2T-DNA InsertionT-DNA is inserted in the reading frame encoding the DYW domainHomozygousSeedlings of mutant line mef35-2 initially develop a little slower in comparison to the WT col plantlets, but look similar at the flowering stageNANADirect Sequencing of PCR Products0.00%UneditedAbsent26470017
SALK_060464mef35-2+MEF35ComplementationThe MEF35 NT (wt) Col reading frame is transformed under control of the 35S promoter in vector Pmdc123HomozygousRetarded growthNANADirect Sequencing of PCR ProductsNAHighRestored26470017
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq94.57%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract96.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Last update: Jul 2021 (version 1.0)