MEF25 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF25
Synonym: MITOCHONDRIAL RNA EDITING FACTOR 25
Description: Encodes a pentatricopeptide repeat protein essential for RNA editing at the nad1-308 site in Arabidopsis mitochondria.
Protein Family: PPR
Subclass: E+
Construct Structure: PLS-E-E+
Gene ID & Species: AT3G25060 (Arabidopsis thaliana)
Edited Gene(s): nad1
Editing Type(s): C-to-U (7)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] Contiguous RNA editing sites in the mitochondrial nad1 transcript of Arabidopsis thaliana are recognized by different proteins, FEBS Letters, 2013. [PMID=23416303]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G25060 Mitochondrion nad1 308 CDS C-to-U CCG/CCG=>CUG/UUG
NA=>NA
P=>L/L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products97.60%HighNA23416303
SAIL 672mef25-1T-DNA InsertionThe T-DNA inserstion causes a 135 bp deletion (between +641 and +775) and a 9 bp insertionHomozygousNo changes were seenLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent23416303
SALK_091381Cmef25-2T-DNA InsertionThe T-DNA inserstion causes a 49 bp deletion (between +936 and +984) and two insertions (8 and 5 bp)HomozygousNo changes were seenLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent23416303
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq95.21%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract20.00%LowDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract19.00%PoorDecreased28761003
Last update: Jul 2021 (version 1.0)