MEF13 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF13
Synonym: MITOCHONDRIAL EDITING FACTOR 13
Description: Encodes an editing factor that requires MORF3 and MORF8 for RNA editing at eight targets in mitochondrial mRNAs in Arabidopsis thaliana.
Protein Family: PPR
Subclass: E,E+
Construct Structure: PLS-E, NA
Gene ID & Species: AT3G02330 (Arabidopsis thaliana)
Edited Gene(s): nad7    nad5    ccmFC    nad2    cox3    nad4
Editing Type(s): C-to-U (49)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] MEF13 Requires MORF3 and MORF8 for RNA Editing at Eight Targets in Mitochondrial mRNAs in Arabidopsis thaliana, Molecular Plant, 2015. [PMID=26048647]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G02330 Mitochondrion ccmFC 50 CDS C-to-U CCU=>CUU
NA=>NA
P=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0No treatmentNo treatmentNo treatmentNo treatmentLeaf3 weeksDirect Sequencing of PCR Products70.00%HighNone26048647
Col-0mef13-1EMS MutantThe EMS mutant causes a single nucleotide (and concomitantamino acid) alteration in the most N-terminal PPR elementNAInitially slower growth; Later stages of development show no gross defects and are fully fertileLeaf3 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent26048647
Col-0mef13-1+35S:At3g02330ComplementationTransgenic overexpression of the MEF13 gene in the mef13-1 mutantNANALeaf3 weeksDirect Sequencing of PCR Products70.00%HighRestored26048647
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq57.30%MediumNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract94.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract49.00%MediumDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract39.00%LowDecreased28761003
Arabidopsis thaliana AT3G02330 Mitochondrion ccmFC 415 CDS C-to-U CUC=>UUC
NA=>NA
L=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0No treatmentNo treatmentNo treatmentNo treatmentLeaf3 weeksDirect Sequencing of PCR Products100.00%CompleteNone26048647
Col-0mef13-1EMS MutantThe EMS mutant causes a single nucleotide (and concomitantamino acid) alteration in the most N-terminal PPR elementNAInitially slower growth; Later stages of development show no gross defects and are fully fertileLeaf3 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent26048647
Col-0mef13-1+35S:At3g02330ComplementationTransgenic overexpression of the MEF13 gene in the mef13-1 mutantNANALeaf3 weeksDirect Sequencing of PCR Products100.00%CompleteRestored26048647
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq58.98%MediumNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract97.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract21.00%LowDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract23.00%LowDecreased28761003
Arabidopsis thaliana AT3G02330 Mitochondrion cox3 314 CDS C-to-U UCU=>UUU
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0No treatmentNo treatmentNo treatmentNo treatmentLeaf3 weeksDirect Sequencing of PCR Products100.00%CompleteNone26048647
Col-0mef13-1EMS MutantThe EMS mutant causes a single nucleotide (and concomitantamino acid) alteration in the most N-terminal PPR elementNAInitially slower growth; Later stages of development show no gross defects and are fully fertileLeaf3 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent26048647
Col-0mef13-1+35S:At3g02330ComplementationTransgenic overexpression of the MEF13 gene in the mef13-1 mutantNANALeaf3 weeksDirect Sequencing of PCR Products100.00%CompleteRestored26048647
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq98.55%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract26.00%LowDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract21.00%LowDecreased28761003
Arabidopsis thaliana AT3G02330 Mitochondrion nad2 59 CDS C-to-U UCC=>UUC
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0No treatmentNo treatmentNo treatmentNo treatmentLeaf3 weeksDirect Sequencing of PCR Products100.00%CompleteNone26048647
Col-0mef13-1EMS MutantThe EMS mutant causes a single nucleotide (and concomitantamino acid) alteration in the most N-terminal PPR elementNAInitially slower growth; Later stages of development show no gross defects and are fully fertileLeaf3 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent26048647
Col-0mef13-1+35S:At3g02330ComplementationTransgenic overexpression of the MEF13 gene in the mef13-1 mutantNANALeaf3 weeksDirect Sequencing of PCR Products100.00%CompleteRestored26048647
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq98.41%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract70.00%HighDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract43.00%MediumDecreased28761003
Arabidopsis thaliana AT3G02330 Mitochondrion nad4 158 CDS C-to-U CCU=>CUU
NA=>NA
P=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0No treatmentNo treatmentNo treatmentNo treatmentLeaf3 weeksDirect Sequencing of PCR Products100.00%CompleteNone26048647
Col-0mef13-1EMS MutantThe EMS mutant causes a single nucleotide (and concomitantamino acid) alteration in the most N-terminal PPR elementNAInitially slower growth; Later stages of development show no gross defects and are fully fertileLeaf3 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent26048647
Col-0mef13-1+35S:At3g02330ComplementationTransgenic overexpression of the MEF13 gene in the mef13-1 mutantNANALeaf3 weeksDirect Sequencing of PCR Products100.00%CompleteRestored26048647
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq99.09%HighNone32098170
Arabidopsis thaliana AT3G02330 Mitochondrion nad5 1665 CDS C-to-U CUC=>CUU
NA=>NA
L=>L
NA=>NA
Synonymous
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0No treatmentNo treatmentNo treatmentNo treatmentLeaf3 weeksDirect Sequencing of PCR Products20.00%LowNone26048647
Col-0mef13-1EMS MutantThe EMS mutant causes a single nucleotide (and concomitantamino acid) alteration in the most N-terminal PPR elementNAInitially slower growth; Later stages of development show no gross defects and are fully fertileLeaf3 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent26048647
Col-0mef13-1+35S:At3g02330ComplementationTransgenic overexpression of the MEF13 gene in the mef13-1 mutantNANALeaf3 weeksDirect Sequencing of PCR Products60.00%HighIncreased26048647
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq8.15%PoorNone32098170
Arabidopsis thaliana AT3G02330 Mitochondrion nad5 1916 CDS C-to-U UCU=>UUU
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0Col-0No treatmentNo treatmentNo treatmentNo treatmentLeaf3 weeksDirect Sequencing of PCR Products100.00%CompleteNone26048647
Col-0mef13-1EMS MutantThe EMS mutant causes a single nucleotide (and concomitantamino acid) alteration in the most N-terminal PPR elementNAInitially slower growth; Later stages of development show no gross defects and are fully fertileLeaf3 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent26048647
Col-0mef13-1+35S:At3g02330ComplementationTransgenic overexpression of the MEF13 gene in the mef13-1 mutantNANALeaf3 weeksDirect Sequencing of PCR Products100.00%CompleteRestored26048647
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq99.62%HighNone32098170
Arabidopsis thaliana AT3G02330 Mitochondrion nad7 213 CDS C-to-U GAC=>GAU
NA=>NA
D=>D
NA=>NA
Synonymous
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
C24C24No treatmentNo treatmentNo treatmentNo treatmentLeaf3 weeksDirect Sequencing of PCR Products70.00%HighNone26048647
Col-0Col-0No treatmentNo treatmentNo treatmentNo treatmentLeaf3 weeksDirect Sequencing of PCR Products100.00%CompleteNone26048647
Col-0mef13-1EMS MutantThe EMS mutant causes a single nucleotide (and concomitantamino acid) alteration in the most N-terminal PPR elementNAInitially slower growth; Later stages of development show no gross defects and are fully fertileLeaf3 weeksDirect Sequencing of PCR Products0.00%UneditedAbsent26048647
Col-0mef13-1+35S:At3g02330ComplementationTransgenic overexpression of the MEF13 gene in the mef13-1 mutantNANALeaf3 weeksDirect Sequencing of PCR Products100.00%CompleteRestored26048647
SALK_097270Cmef13-2T-DNA InsertionT-DNA is inserted at the C terminus of the E domain which removes the C-terminal 67 amino acidsNANALeaf3 weeksDirect Sequencing of PCR Products100.00%CompleteRestored26048647
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq91.31%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract51.00%MediumDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract21.00%LowDecreased28761003
Last update: Jul 2021 (version 1.0)