CRR22 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT1G11290

Chloroplast
Plastid

ndhB

746

CDS

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Nössen	crr22-1	Ds Insertion	NA	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nössen	crr22-1+CRR22DYW2	Complementation	crr22-1 is transformed with CRR22, in which the DYW motif is replaced by that of CRR2	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
Nössen	crr22-1+CRR22DYW28	Complementation	crr22-1 is transformed with CRR22, in which the DYW motif is replaced by that of CRR28	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Nössen	crr22-1+CRR22ΔDYW	Complementation	crr22-1 is transformed with CRR22 lacking the DYW motif	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Nössen	crr22-1+CRR22ΔE/DYW	Complementation	crr22-1 is transformed with CRR22 lacking the E and DYW motifs	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
Nössen	crr22-2	Ds Insertion	NA	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nössen	crr22-3	Ds Insertion	The Ds transposon is inserted into 39-untranslated region sequences	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nössen	Nössen	No treatment	No treatment	No treatment	No treatment	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	19182104
NA	NA	Gel mobility shift (GMS) assays	NA	NA	NA	NA	NA	NA	NA	NA	NA	22362750
Col-0	WT	No treatment	No treatment	No treatment	NA	NA	NA	RNA-seq	93.52%	High	None	32098170
Col-0	WT	No treatment	No treatment	No treatment	Normal	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	97.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003

Arabidopsis thaliana

AT1G11290

Chloroplast
Plastid

ndhD

887

CDS

C-to-U

CCC=>CUC
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Nössen	crr22-1	Ds Insertion	NA	Recessive mutation	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nössen	crr22-1+CRR22DYW2	Complementation	crr22-1 is transformed with CRR22, in which the DYW motif is replaced by that of CRR2	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
Nössen	crr22-1+CRR22DYW28	Complementation	crr22-1 is transformed with CRR22, in which the DYW motif is replaced by that of CRR28	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Nössen	crr22-1+CRR22ΔDYW	Complementation	crr22-1 is transformed with CRR22 lacking the DYW motif	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Nössen	crr22-1+CRR22ΔE/DYW	Complementation	crr22-1 is transformed with CRR22 lacking the E and DYW motifs	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
Nössen	crr22-2	Ds Insertion	NA	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nössen	crr22-3	Ds Insertion	The Ds transposon is inserted into 39-untranslated region sequences	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nössen	Nössen	No treatment	No treatment	No treatment	No treatment	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	19182104
NA	NA	Gel mobility shift (GMS) assays	NA	NA	NA	NA	NA	NA	NA	NA	NA	22362750
Col-0	WT	No treatment	No treatment	No treatment	NA	NA	NA	RNA-seq	82.87%	High	None	32098170
Col-0	WT	No treatment	No treatment	No treatment	Normal	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	66.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	84.00%	High	Increased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	96.00%	High	Increased	28761003

Arabidopsis thaliana

AT1G11290

Chloroplast
Plastid

rpoB

551

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Nössen	crr22-1	Ds Insertion	NA	Recessive mutation	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nössen	crr22-1+CRR22DYW2	Complementation	crr22-1 is transformed with CRR22, in which the DYW motif is replaced by that of CRR2	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
Nössen	crr22-1+CRR22DYW28	Complementation	crr22-1 is transformed with CRR22, in which the DYW motif is replaced by that of CRR28	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Nössen	crr22-1+CRR22ΔDYW	Complementation	crr22-1 is transformed with CRR22 lacking the DYW motif	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Nössen	crr22-1+CRR22ΔE/DYW	Complementation	crr22-1 is transformed with CRR22 lacking the E and DYW motifs	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
Nössen	crr22-2	Ds Insertion	NA	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nössen	crr22-3	Ds Insertion	The Ds transposon is inserted into 39-untranslated region sequences	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Nössen	Nössen	No treatment	No treatment	No treatment	No treatment	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	19182104
NA	NA	Gel mobility shift (GMS) assays	NA	NA	NA	NA	NA	NA	NA	NA	NA	22362750
Col-0	WT	No treatment	No treatment	No treatment	NA	NA	NA	RNA-seq	82.62%	High	None	32098170
Col-0	WT	No treatment	No treatment	No treatment	Normal	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	95.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	95.00%	High	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	93.00%	High	Similar	28761003

Editing Factor:	CRR22
Synonym:	CHLORORESPIRATORY REDUCTION22
Description:	Encodes a PPR-DYW protein required for editing of multiple plastid transcripts.
Protein Family:	PPR
Subclass:	DYW
Construct Structure:	PLS-E-DYW
Gene ID & Species:	AT1G11290 (Arabidopsis thaliana)
Edited Gene(s):	ndhB ndhD rpoB
Editing Type(s):	C-to-U (39)
Publication(s):	[1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170] [2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003] [3] A pentatricopeptide repeat protein acts as a site-specificity factor at multiple RNA editing sites with unrelated cis-acting elements in plastids, Nucleic Acids Research, 2012. [PMID=22362750] [4] Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts, The Plant Cell, 2009. [PMID=19182104]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details