Standards
1.3.2.3 Strategy

Strategy:sequencing technique intended for the library.

Strategy Sequencing strategy used in the experiment
WGA Whole genome amplification.
WGS Whole genome shotgun.
WES Whole exome sequencing is a genomic technique for sequencing, all of the protein-coding genes in a genome (known as the exome).
WXS Random sequencing of exonic regions selected from the genome.
RNA-Seq Random sequencing of whole transcriptome.
miRNA-Seq Micro RNA and other small non-coding RNA sequencing.
Tn-Seq Gene fitness determination through transposon seeding.
WCS Whole chromosome (or other replicon) shotgun.
CLONE Genomic clone based (hierarchical) sequencing.
POOLCLONE Shotgun of pooled clones (usually BACs and Fosmids).
AMPLICON Sequencing of overlapping or distinct PCR or RT-PCR products.
CLONEEND Clone end (5', 3', or both) sequencing.
FINISHING Sequencing intended to finish (close) gaps in existing coverage.
ChIP-Seq Direct sequencing of chromatin immunoprecipitates.
MNase-Seq Direct sequencing following MNase digestion.
DNase-Hypersensitivity Sequencing of hypersensitive sites, or segments of open chromatin that are more readily cleaved by DNaseI.
Bisulfite-Seq Sequencing following treatment of DNA with bisulfite to convert cytosine residues to uracil depending on methylation status.
EST Single pass sequencing of cDNA templates.
FL-cDNA Full-length sequencing of cDNA templates.
CTS Concatenated Tag Sequencing.
MRE-Seq Methylation-Sensitive Restriction Enzyme Sequencing strategy.
MeDIP-Seq Methylated DNA Immunoprecipitation Sequencing strategy.
MBD-Seq Direct sequencing of methylated fractions sequencing strategy.
Synthetic-Long-Read Binning and barcoding of large DNA fragments to facilitate assembly of the fragment.
ATAC-seq Assay for Transposase-Accessible Chromatin (ATAC) strategy is used to study genome-wide chromatin accessibility. alternative method to DNase-seq that uses an engineered Tn5 transposase to cleave DNA and to integrate primer DNA sequences into the cleaved genomic DNA.
ChIA-PET Direct sequencing of proximity-ligated chromatin immuneprecipitates.
FAIRE-seq Formaldehyde Assisted Isolation of Regulatory Elements.
Hi-C Chromosome Conformation Capture technique where a biotinlabeled nucleotide is incorporated at the ligation junction, enabling selective purification of chimeric DNA ligation junctions followed by deep sequencing.
ncRNA-Seq Capture of other non-coding RNA types, including post-translation modification types such as snRNA (small nuclear RNA) or snoRNA (small nucleolar RNA), or expression regulation types such as siRNA (small interfering RNA) or piRNA/piwi/RNA (piwi-interacting RNA).
RAD-Seq Restriction Site Associated DNA Sequence.
RIP-Seq Direct sequencing of RNA immunoprecipitates (includes CLIP-Seq, HITS-CLIP and PAR-CLIP).
SELEX Systematic Evolution of Ligands by EXponential enrichment.
ssRNA-seq strand-specific RNA sequencing.
Targeted-Capture Targeted-Capture sequencing.
Tethered Chromatin Conformation Capture Tethered Chromatin Conformation Capture sequencing.
TCR-seq High throughput sequencing to map T-cell receptor (TCR) repertoires at high resolution.
BCR-seq High throughput sequencing to map B-cell receptor (BCR) repertoires at high resolution.
MeRIP-Seq MeRIP-Seq maps m6A-methylated RNA. Deep sequencing provides high-resolution reads of m6A-methylated RNA.
OTHER Library strategy not listed (please include additional info in the “design description”).