Source:The library source specifies the type of source material that is being sequenced.
Source | Type of genetic source material sequenced |
---|---|
GENOMIC | Genomic DNA (includes PCR products from genomic DNA). |
TRANSCRIPTOMIC | Transcription products or non-genomic DNA (EST, cDNA, RT-PCR, screened libraries. |
METATRANSCRIPTOMIC | Transcription products from community targets. |
METAGENOMIC | Mixed material from metagenome. |
SYNTHETIC | Synthetic DNA. |
VIRAL RNA | Viral RNA. |
OTHER | Other, unspecified, or unknown library source material. (please include additional info in the “design description”) |
Selection:whether any method was used to select and/or enrich the material being sequenced.
Selection | Method of selection or enrichment used in the Experiment |
unspecified | Library enrichment, screening, or selection is not specified. (please include additional info in the “design description”) |
RANDOM | Random selection by shearing or other method. |
PCR | Source material was selected by designed primers. |
RANDOM PCR | Source material was selected by randomly generated primers. |
RT-PCR | Source material was selected by reverse transcription PCR. |
HMPR | Hypo-methylated partial restriction digest. |
MF | Methyl Filtrated. |
CF-S | Cot-filtered single/low-copy genomic DNA. |
CF-M | Cot-filtered moderately repetitive genomic DNA. |
CF-H | Cot-filtered highly repetitive genomic DNA. |
CF-T | Cot-filtered theoretical single-copy genomic DNA. |
MDA | Multiple displacement amplification. |
MSLL | Methylation Spanning Linking Library. |
cDNA | complementary DNA. |
ChIP | Chromatin immunoprecipitation. |
MNase | Micrococcal Nuclease (MNase) digestion. |
DNAse | Deoxyribonuclease (MNase) digestion. |
Hybrid Selection | Selection by hybridization in array or solution. |
Reduced Representation | Reproducible genomic subsets, often generated by restriction fragment size selection, containing a manageable number of loci to facilitate re-sampling. |
Restriction Digest | DNA fractionation using restriction enzymes. |
5-methylcytidine antibody | Selection of methylated DNA fragments using an antibody raised against 5-methylcytosine or 5-methylcytidine (m5C). |
MBD2 protein methyl-CpG binding domain | Enrichment by methyl-CpG binding domain. |
CAGE | Cap-analysis gene expression. |
RACE | Rapid Amplification of cDNA Ends. |
size fractionation | Physical selection of size appropriate targets. |
Padlock probes capture method | Circularized oligonucleotide probes. |
Poly-A | polyA enriched RNA-seq. |
other | Other library enrichment, screening, or selection process. (please include additional info in the “design description”) |