1.3.2.4 Source

Source:The library source specifies the type of source material that is being sequenced.

Source	Type of genetic source material sequenced
GENOMIC	Genomic DNA (includes PCR products from genomic DNA).
TRANSCRIPTOMIC	Transcription products or non-genomic DNA (EST, cDNA, RT-PCR, screened libraries.
METATRANSCRIPTOMIC	Transcription products from community targets.
METAGENOMIC	Mixed material from metagenome.
SYNTHETIC	Synthetic DNA.
VIRAL RNA	Viral RNA.
OTHER	Other, unspecified, or unknown library source material. (please include additional info in the “design description”)

1.3.2.5 Selection

Selection:whether any method was used to select and/or enrich the material being sequenced.

Selection	Method of selection or enrichment used in the Experiment
unspecified	Library enrichment, screening, or selection is not specified. (please include additional info in the “design description”)
RANDOM	Random selection by shearing or other method.
PCR	Source material was selected by designed primers.
RANDOM PCR	Source material was selected by randomly generated primers.
RT-PCR	Source material was selected by reverse transcription PCR.
HMPR	Hypo-methylated partial restriction digest.
MF	Methyl Filtrated.
CF-S	Cot-filtered single/low-copy genomic DNA.
CF-M	Cot-filtered moderately repetitive genomic DNA.
CF-H	Cot-filtered highly repetitive genomic DNA.
CF-T	Cot-filtered theoretical single-copy genomic DNA.
MDA	Multiple displacement amplification.
MSLL	Methylation Spanning Linking Library.
cDNA	complementary DNA.
ChIP	Chromatin immunoprecipitation.
MNase	Micrococcal Nuclease (MNase) digestion.
DNAse	Deoxyribonuclease (MNase) digestion.
Hybrid Selection	Selection by hybridization in array or solution.
Reduced Representation	Reproducible genomic subsets, often generated by restriction fragment size selection, containing a manageable number of loci to facilitate re-sampling.
Restriction Digest	DNA fractionation using restriction enzymes.
5-methylcytidine antibody	Selection of methylated DNA fragments using an antibody raised against 5-methylcytosine or 5-methylcytidine (m5C).
MBD2 protein methyl-CpG binding domain	Enrichment by methyl-CpG binding domain.
CAGE	Cap-analysis gene expression.
RACE	Rapid Amplification of cDNA Ends.
size fractionation	Physical selection of size appropriate targets.
Padlock probes capture method	Circularized oligonucleotide probes.
Poly-A	polyA enriched RNA-seq.
other	Other library enrichment, screening, or selection process. (please include additional info in the “design description”)