Experiment information
Accession CRX223973
Organism soil metagenome
Title F-TS4-3
BioProject PRJCA001644
BioSample SAMC338340
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total fungi genomic DNA samples were extracted using DNA extraction kit for the corresponding sample. The concentration and purity were measured using the NanoDrop One. The ITS2 region of 16S rRNA in fungi was amplified by PCR with forward primer ITS3F and reverse primer ITS4R. Sample-specific 12-bp barcode sequences was added to the same DNA library to distinguish different samples. Primers were synthesized by Invitrogen. At last, the library was sequenced on an IlluminaHiseq2500 platform and 258 bp paired-end reads were generated. AMPLICON METAGENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 450
Planned read length (bp) for mate 2: 450
Release date2021-02-10
Run
Run accession Run data file information
File nameFile size (MB)
CRR266072 CRR266072_f1.fq.gz
CRR266072_r2.fq.gz
7.34
8.27
SubmitterDianming Wu (dmwu@geo.ecnu.edu.cn)
OrganizationEast China Normal University
Date submitted2021-02-10