Accession | CRX223968 |
Organism | soil metagenome |
Title | F-TP2S2-1 |
BioProject | PRJCA001644 |
BioSample | SAMC338335 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total fungi genomic DNA samples were extracted using DNA extraction kit for the corresponding sample. The concentration and purity were measured using the NanoDrop One. The ITS2 region of 16S rRNA in fungi was amplified by PCR with forward primer ITS3F and reverse primer ITS4R. Sample-specific 12-bp barcode sequences was added to the same DNA library to distinguish different samples. Primers were synthesized by Invitrogen. At last, the library was sequenced on an IlluminaHiseq2500 platform and 253 bp paired-end reads were generated. |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 450
Planned read length (bp) for mate 2: 450
|
Release date | 2021-02-10 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR266067 |
CRR266067_f1.fq.gz
CRR266067_r2.fq.gz
|
5.38
5.94
|
|
Submitter | Dianming Wu (dmwu@geo.ecnu.edu.cn) |
Organization | East China Normal University |
Date submitted | 2021-02-10 |