| Accession | CRX223964 |
| Organism | soil metagenome |
| Title | A-NTS4-3 |
| BioProject | PRJCA001644 |
| BioSample | SAMC338331 |
| Platform | Illumina HiSeq 2500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total archaea genomic DNA samples were extracted using DNA extraction kit for the corresponding sample. The concentration and purity were measured using the NanoDrop One. The V4-V5 region of 16S rRNA in archaea was amplified by PCR with forward primer 519F and reverse primer 915R. Sample-specific 12-bp barcode sequences was added to the same DNA library to distinguish different samples. The library was sequenced on an IlluminaHiseq2500 platform and 267 bp paired-end reads were generated. |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 400
Planned read length (bp) for mate 2: 400
|
| Release date | 2021-02-10 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR266063 |
CRR266063_f1.fq.gz
CRR266063_r2.fq.gz
|
3.34
4.07
|
|
| Submitter | Dianming Wu (dmwu@geo.ecnu.edu.cn) |
|---|
| Organization | East China Normal University |
|---|
| Date submitted | 2021-02-10 |
|---|
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