Accession | CRX223963 |
Organism | soil metagenome |
Title | A-NTS4-2 |
BioProject | PRJCA001644 |
BioSample | SAMC338330 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total archaea genomic DNA samples were extracted using DNA extraction kit for the corresponding sample. The concentration and purity were measured using the NanoDrop One. The V4-V5 region of 16S rRNA in archaea was amplified by PCR with forward primer 519F and reverse primer 915R. Sample-specific 12-bp barcode sequences was added to the same DNA library to distinguish different samples. The library was sequenced on an IlluminaHiseq2500 platform and 266 bp paired-end reads were generated. |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 400
Planned read length (bp) for mate 2: 400
|
Release date | 2021-02-10 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR266062 |
CRR266062_f1.fq.gz
CRR266062_r2.fq.gz
|
3.02
3.68
|
|
Submitter | Dianming Wu (dmwu@geo.ecnu.edu.cn) |
Organization | East China Normal University |
Date submitted | 2021-02-10 |