Accession | CRX203220 |
Organism | Nicotiana tabacum |
Title | Experiment 2 |
BioProject | PRJCA004174 |
BioSample | SAMC308866 |
Platform | Illumina NovaSeq 6000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
After RNA sample quality control, high-quality RNA was obtained and used for library construction. The pipeline was consisted of five main steps: (1) Isolated mRNA from total RNA using the oligo(dT) magnetic beads; (2) Mixed with the ragmentation buffer and fragment the mRNA randomly; (3) Using mRNA as the template, six-base random primers (random hexamers) were used to synthesize the first strand cDNA. Then PCR buffer, dNTPs, RNase H and DNA polymerase I were added to do second strand cDNA synthesis. AMPure XP beads are used to purify cDNAs; (4) Purified double-stranded cDNAs were resolved with EB buffer for end reparation and single nucleotide A addition. Then AMPure XP beads were used in size selection; (5) Finally, cDNA sequencing library was obtained by PCR amplification. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
Release date | 2021-01-13 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR241561 |
CRR241561_f1.fq.gz
CRR241561_r2.fq.gz
|
1,239.39
1,310.92
|
|
Submitter | Zhang Yanzhao (landscape031@163.com) |
Organization | Luoyang Normal University |
Date submitted | 2021-01-13 |