Experiment information
Accession CRX203219
Organism Nicotiana tabacum
Title Experiment 1
BioProject PRJCA004174
BioSample SAMC308865
Platform Illumina NovaSeq 6000
Library
Library name Construction protocol Strategy Source Selection Layout
After RNA sample quality control, high-quality RNA was obtained and used for library construction. The pipeline was consisted of five main steps: (1) Isolated mRNA from total RNA using the oligo(dT) magnetic beads; (2) Mixed with the ragmentation buffer and fragment the mRNA randomly; (3) Using mRNA as the template, six-base random primers (random hexamers) were used to synthesize the first strand cDNA. Then PCR buffer, dNTPs, RNase H and DNA polymerase I were added to do second strand cDNA synthesis. AMPure XP beads are used to purify cDNAs; (4) Purified double-stranded cDNAs were resolved with EB buffer for end reparation and single nucleotide A addition. Then AMPure XP beads were used in size selection; (5) Finally, cDNA sequencing library was obtained by PCR amplification. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2021-01-13
Run
Run accession Run data file information
File nameFile size (MB)
CRR241560 CRR241560_f1.fq.gz
CRR241560_r2.fq.gz
1,411.59
1,485.3
SubmitterZhang Yanzhao (landscape031@163.com)
OrganizationLuoyang Normal University
Date submitted2021-01-13
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Experiments(5)