Experiment information
Accession CRX166582
Organism Epinephelus lanceolatus
Title RNA-seq data
BioProject PRJCA003546
BioSample SAMC251390
Platform Illumina HiSeq 2000
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA of the 10 tissues was extracted using RNAiso reagents (Takara, China) following the manufacturer’s instructions. The quantity and quality of RNA samples were determined using a microplate spectrophotometer (BioTek Company, USA) and electrophoresis was conducted using 1% agarose gel. Total RNA of the 10 tissues was mixed at equal amounts to generate a mixed RNA pool. An RNA-seq library was prepared based on RNA-seq libraries were prepared using NEBNext UltraTM RNA Library Prep Kit (NEB, USA) following the manufacturer’s protocols. The library quality and quantity was measured using Agilent 2100 Bioanalyzer (Agilent Technologies). RNA-Seq TRANSCRIPTOMIC RANDOM PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2020-10-15
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR194609 2020-10-15 andaishibanyu_S390-04-T01_good_1.fq.gz
andaishibanyu_S390-04-T01_good_2.fq.gz
2,179.96
2,279.17
Submitteryang yang (214453598@qq.com)
OrganizationsSun Yat-sen University
Date submitted2020-10-10
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