| Accession | CRX166582 |
| Organism | Epinephelus lanceolatus |
| Title | RNA-seq data |
| BioProject | PRJCA003546 |
| BioSample | SAMC251390 |
| Platform | Illumina HiSeq 2000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA of the 10 tissues was extracted using RNAiso reagents (Takara, China) following the manufacturer’s instructions. The quantity and quality of RNA samples were determined using a microplate spectrophotometer (BioTek Company, USA) and electrophoresis was conducted using 1% agarose gel. Total RNA of the 10 tissues was mixed at equal amounts to generate a mixed RNA pool. An RNA-seq library was prepared based on RNA-seq libraries were prepared using NEBNext UltraTM RNA Library Prep Kit (NEB, USA) following the manufacturer’s protocols. The library quality and quantity was measured using Agilent 2100 Bioanalyzer (Agilent Technologies). |
RNA-Seq |
TRANSCRIPTOMIC |
RANDOM |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2020-10-15 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR194609 |
CRR194609_f1.fq.gz
CRR194609_r2.fq.gz
|
2,179.96
2,279.17
|
|
| Submitter | yang yang (214453598@qq.com) |
|---|
| Organization | Sun Yat-sen University |
|---|
| Date submitted | 2020-10-10 |
|---|
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