| Accession | CRX166581 |
| Organism | Epinephelus lanceolatus |
| Title | HiC-data |
| BioProject | PRJCA003546 |
| BioSample | SAMC251389 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Muscle samples were fixed with fresh paraformaldehyde and then DNA-protein bonds were created. The Mbo I restriction enzyme was used to digest the DNA and the overhanging 5' ends of the DNA fragments were repaired with a biotinylated residue. The fragments close to each other in the nucleus during fixation were ligated and the denatured proteins were removed. The Hi-C fragments were further sheared by sonication, which were then pulled down with streptavidin beads. The library was sequenced on the Illumina HiSeq X Ten platform |
Hi-C |
GENOMIC |
RANDOM |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
| Release date | 2020-10-16 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR194608 |
CRR194608_f1.fq.gz
CRR194608_r2.fq.gz
|
23,011.39
24,760.29
|
|
| Submitter | yang yang (214453598@qq.com) |
|---|
| Organization | Sun Yat-sen University |
|---|
| Date submitted | 2020-10-10 |
|---|
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