Experiment information
Accession CRX153042
Organism Paeonia suffruticosa
Title Exp.stage01-1
BioProject PRJCA000701
BioSample SAMC238037
Platform BGISEQ-500
Library
Library name Construction protocol Strategy Source Selection Layout
The poly-A-containing mRNA was purified using oligo (dT) magnetic beads, and then cleaved into small pieces using fragmentation buffer. The first cDNA strand was synthesized using random hexamer primers and reverse transcriptase, followed by second-strand cDNA synthesis using DNA polymerase I and RNase H. The short doublestranded fragments were purified with a QiaQuick PCR Purification Kit (Qiagen, CA, USA) and dissolved with the EB buffer supplied in the kit for end preparation and poly(A) addition. The sequence adaptors were linked to two ends of the short cDNA sequences. Agarose gel electrophoresis was used to select suitably sized fragments, and the products were subsequently amplified by PCR. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 101
Planned read length (bp) for mate 2: 101
Release date2020-09-13
Run
Run accession Run data file information
File nameFile size (MB)
CRR180618 CRR180618_f1.fq.gz
CRR180618_r2.fq.gz
3,874.9
3,889.74
SubmitterZhang Yanzhao (landscape031@163.com)
OrganizationLuoyang Normal University
Date submitted2020-09-11