Accession | CRX153042 |
Organism | Paeonia suffruticosa |
Title | Exp.stage01-1 |
BioProject | PRJCA000701 |
BioSample | SAMC238037 |
Platform | BGISEQ-500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The poly-A-containing mRNA was purified using oligo (dT) magnetic beads, and then cleaved into small pieces using fragmentation buffer. The first cDNA strand was synthesized using random hexamer primers and reverse transcriptase, followed by second-strand cDNA synthesis using DNA polymerase I and RNase H. The short doublestranded fragments were purified with a QiaQuick PCR Purification Kit (Qiagen, CA, USA) and dissolved with the EB buffer supplied in the kit for end preparation and
poly(A) addition. The sequence adaptors were linked to two ends of the short cDNA sequences. Agarose gel electrophoresis was used to select suitably sized fragments, and the products were subsequently amplified by PCR. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 101
Planned read length (bp) for mate 2: 101
|
Release date | 2020-09-13 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR180618 |
CRR180618_f1.fq.gz
CRR180618_r2.fq.gz
|
3,874.9
3,889.74
|
|
Submitter | Zhang Yanzhao (landscape031@163.com) |
Organization | Luoyang Normal University |
Date submitted | 2020-09-11 |