Experiment information
Accession CRX152943
Organism Eriobotrya japonica
Title W_CQ_FL
BioProject PRJCA003246
BioSample SAMC237877
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
The Illumina paired-end reads libraries (350bp) were constructed strictly according to the Illumina guidelines as follows: The quality-tested extracted DNA was fragmented using the E210 Covaris instrument (Covaris, Inc., USA) and segments with about 350 bp length were selected on a 3% agarose gel. The selected DNA fragments were then end-repaired, A-tailed and ligated to Illumina compatible adaptors (BioScientific, Austin, TX,USA) using the SPRIWorks Library Preparation System and SPRI TE instrument (Beckman Coulter), then were PCR-amplified by using Illumina adapter-specific primers and Platinum Pfx DNA polymerase (Invitrogen) and the paired-end sequencing library was finished. WGS GENOMIC RANDOM PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Nominal size (bp): 125
Release date2021-02-16
Run
Run accession Run data file information
File nameFile size (MB)
CRR180517 CRR180517_f1.fq.gz
CRR180517_r2.fq.gz
1,575
1,715.01
Submitteryunsheng wang (wys3269@126.com)
OrganizationKaili University
Date submitted2020-09-10