Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The Illumina paired-end reads libraries (350bp) were constructed strictly according to the Illumina guidelines as follows: The quality-tested extracted DNA was fragmented using the E210 Covaris instrument (Covaris, Inc., USA) and segments with about 350 bp length were selected on a 3% agarose gel. The selected DNA fragments were then end-repaired, A-tailed and ligated to Illumina compatible adaptors (BioScientific, Austin, TX,USA) using the SPRIWorks Library Preparation System and SPRI TE instrument (Beckman Coulter), then were PCR-amplified by using Illumina adapter-specific primers and Platinum Pfx DNA polymerase (Invitrogen) and the paired-end sequencing library was finished. |
WGS |
GENOMIC |
RANDOM |
PAIRED
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