Accession | CRX151324 |
Organism | Arabidopsis thaliana |
Title | STARR-seq_cDNA_rep1 |
BioProject | PRJCA003346 |
BioSample | SAMC231916 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Reporter cDNA libraries construction for Illumina sequencing: 24hrs post-transfection, cells were washed in W5 solution twice and concentrated. Total RNA was extracted using TransZolTM Up Plus RNA Kit (ER501). polyA+ RNA fraction was isolated using VAHTS mRNA Capture Beads (N401). 5U DNase I (NEB M0303S) was used to digest DNA at 37°C for 20 min. Synthesis of first strand cDNA was carried out with TransScript One-Step gDNA Removal and cDNA synthesis SuperMix (AU311 50°C for 30 min and 85°C for 5s) using a specific primer (GACTGGAGTTCAGACGTGTGCTCTTCCGATCTttttttttttttttttttNNNNNN) and 8ul of RNA in 20 reactions. All reactions were pooled. The total amount of reporter cDNA was amplified for Illumina sequencing by a 2-step nested PCR strategy using the TransStart® FastPfu Fly DNA Polymerase (AP231-12). |
OTHER |
GENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
Release date | 2020-10-14 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR177590 |
CRR177590_f1.fq.gz
CRR177590_r2.fq.gz
|
9,617.39
11,193.14
|
|
Submitter | Jing Wan (1130014467@qq.com) |
Organization | Huazhong Agricultural University |
Date submitted | 2020-08-31 |