Experiment information
Accession CRX151324
Organism Arabidopsis thaliana
Title STARR-seq_cDNA_rep1
BioProject PRJCA003346
BioSample SAMC231916
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Reporter cDNA libraries construction for Illumina sequencing: 24hrs post-transfection, cells were washed in W5 solution twice and concentrated. Total RNA was extracted using TransZolTM Up Plus RNA Kit (ER501). polyA+ RNA fraction was isolated using VAHTS mRNA Capture Beads  (N401). 5U DNase I (NEB M0303S) was used to digest DNA at 37°C for 20 min. Synthesis of first strand cDNA was carried out with TransScript One-Step gDNA Removal and cDNA synthesis SuperMix (AU311 50°C for 30 min and 85°C for 5s) using a specific primer (GACTGGAGTTCAGACGTGTGCTCTTCCGATCTttttttttttttttttttNNNNNN) and 8ul of RNA in 20 reactions. All reactions were pooled. The total amount of reporter cDNA was amplified for Illumina sequencing by a 2-step nested PCR strategy using the TransStart® FastPfu Fly DNA Polymerase (AP231-12). OTHER GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2020-10-14
Run
Run accession Run data file information
File nameFile size (MB)
CRR177590 CRR177590_f1.fq.gz
CRR177590_r2.fq.gz
9,617.39
11,193.14
SubmitterJing Wan (1130014467@qq.com)
OrganizationHuazhong Agricultural University
Date submitted2020-08-31
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