Experiment information
Accession CRX127140
Organism Populus alba
Title S10_S_2
BioProject PRJCA003153
BioSample SAMC202590
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Isolated mRNA from total RNA using the oligo(dT) magnetic beads;Mixed with the fragmentation buffer and fragment the mRNA randomly;Using mRNA as the template, six-base random primers (random hexamers) were used to synthesize the first strand cDNA. Then PCR buffer, dNTPs, RNase H and DNA polymerase I were added to do second strand cDNA synthesis. AMPure XP beads are used to purify cDNAs;Purified double-stranded cDNAs were resolved with EB buffer for end reparation and single nucleotide A addition. Then AMPure XP beads were used in size selection;Finally, cDNA sequencing library was obtained by PCR amplification. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2020-08-04
Run
Run accession Run data file information
File nameFile size (MB)
CRR153286 CRR153286_f1.fq.gz
CRR153286_r2.fq.gz
1,566.48
1,638.62
SubmitterChengming Fan (cmfan@genetics.ac.cn)
OrganizationInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences
Date submitted2020-07-31