Accession | CRX127127 |
Organism | Populus alba |
Title | S0_R_1 |
BioProject | PRJCA003153 |
BioSample | SAMC202577 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Isolated mRNA from total RNA using the oligo(dT) magnetic beads;Mixed with the fragmentation buffer and fragment the mRNA randomly;Using mRNA as the template, six-base random primers (random hexamers) were used to synthesize the first strand cDNA. Then PCR buffer, dNTPs, RNase H and DNA polymerase I were added to do second strand cDNA synthesis. AMPure XP beads are used to purify cDNAs;Purified double-stranded cDNAs were resolved with EB buffer for end reparation and single nucleotide A addition. Then AMPure XP beads were used in size selection;Finally, cDNA sequencing library was obtained by PCR amplification. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
|
Release date | 2020-08-04 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR153273 |
CRR153273_f1.fq.gz
CRR153273_r2.fq.gz
|
1,492.53
1,562.08
|
|
Submitter | Chengming Fan (cmfan@genetics.ac.cn) |
Organization | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences |
Date submitted | 2020-07-31 |