Experiment information
Accession CRX126936
Organism Norovirus
Title 43374
BioProject PRJCA003123
BioSample SAMC202432
Platform Illumina MiSeq
Library
Library name Construction protocol Strategy Source Selection Layout
The nucleic acid was extracted from the 140 ul concentrated sewage. Reverse transcription (RT)-PCR was performed by using random primers. The cDNA was then used for two rounds of PCR amplification with specific primer sets. The positive products (~354 nucleotides) were subjected to Illumina MiSeq sequencing (PE250) and around 1,000,000 reads were generated for each PCR product. AMPLICON VIRAL RNA PCR PAIRED
Processing Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Release date2020-08-04
Run
Run accession Run data file information
File nameFile size (MB)
CRR153080 CRR153080_f1.fq.gz
CRR153080_r2.fq.gz
69.95
75.8
SubmitterJing Lu (jimlu0331@163.com)
OrganizationGuangdong Provincial Center for Diseases Control and prevention
Date submitted2020-07-29