Experiment information
Accession CRX126672
Organism Severe acute respiratory syndrome coronavirus 2
Title siCTRL_SARS-CoV-2_T_rep2
BioProject PRJCA003106
BioSample SAMC201973
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Cultured cells were washed once with PBS and then subjected to RNA purification with TRIzol (ThermoFisher). Purified total RNAs were first treated with Turbo DNase (ThermoFisher) and then depleted the rRNAs with the Ribo-off rRNA Depletion Kit (Human/Mouse/Rat) (Vazyme) following the manufacturer’s instructions. RNA-Seq libraries were directly constructed using the KAPA RNA HyperPrep Kit (KAPA Biosystems, KK8541) following the manufacturer’s instructions. RNA-Seq TRANSCRIPTOMIC size fractionation PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Release date2021-01-01
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR152707 2021-01-01 siCTRL_SARS-CoV-2_T_rep2_1.fq.gz
siCTRL_SARS-CoV-2_T_rep2_2.fq.gz
3,431.33
3,696.2
SubmitterGao chun chun (gaochunchun@big.ac.cn)
OrganizationsBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2020-07-27
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