Experiment information
Accession CRX125648
Organism Bacteria
Title PecQ1
BioProject PRJCA003098
BioSample SAMC200964
Platform Illumina MiSeq
Library
Library name Construction protocol Strategy Source Selection Layout
The total DNA was extracted using improved CTAB method and purified using a DNA fast purifying kit. The V4 - V5 hypervariable region of the 16S rRNA gene was amplified using the 515F and 909R primers according to previous reports. PCR production was purified using a SanPrep gel DNA extraction kit. All purified PCR productions were pooled together with an equal molar amount from each sample and sequencing using MiSeq system. AMPLICON METAGENOMIC PCR SINGLE
Processing Planned read length (bp): 420
Release date2020-07-23
Run
Run accession Run data file information
File nameFile size (MB)
CRR151768 CRR151768.fastq.gz 17.89
SubmitterLutian Mao (mlt@hzu.edu.cn)
OrganizationHuizhou University
Date submitted2020-07-22