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Experiment information

Accession

CRX125620

Organism

Title

ZJX-A4560

BioProject

BioSample

Platform

Illumina NovaSeq 5000

Library

Library name	Construction protocol	Strategy	Source	Selection	Layout
	Total RNA was isolated from samples using the Trizol reagent (Invitrogen, USA) according to the manufacturer’s instructions. RNA quality and quantity were determined with a spectrophotometer (IMPLEN, Germany) and an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). RNA-seq was performed at the Novogene Company (Beijing, China). The RNA-Seq library was constructed using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. The cleaved RNA fragments were transcribed into first-strand cDNA using reverse transcriptase and subsequently second-strand cDNA synthesis was performed using DNA polymerase I and RNase H. The fragments were ligated to sequencing adaptors and the library preparations were sequenced on an Illumina HiSeq 4000 platform and 152 bp paired-end reads were generated.	RNA-Seq	TRANSCRIPTOMIC	PolyA	PAIRED

Processing

Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350

Release date

2020-07-23

Run

Run accession	Run data file information
Run accession	File name	File size (MB)
CRR151740	CRR151740_f1.fq.gz CRR151740_r2.fq.gz	1,742.56 1,779.28

Submitter

Jingxiong Zhang (zhangjingxiong@mail.kib.ac.cn)

Organization

Chinese Academy of Sciences

Date submitted

2020-07-22

Related experiments

Experiments(5)