Accession | CRX125020 |
Organism | Mus musculus |
Title | Dynamic profiles and transcriptional preferences of histone modifications during spermiogenesis |
BioProject | PRJCA003045 |
BioSample | SAMC199710 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Single-cell suspensions with 1×10^5 cells/mL in concentration in PBS (HyClone) were prepared. Single-cell suspensions were then loaded onto microfluidic devices and scRNA-seq libraries were constructed according to Singleron GEXSCOPETM protocol by GEXSCOPETM Single-Cell RNA Library Kit (Singleron Biotechnologies) . Individual libraries were diluted to 4nM and pooled for sequencing. Pools were sequenced on Illumina HiSeq X with 150 bp paired end reads. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
|
Release date | 2020-07-15 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR151127 |
CRR151127_f1.fq.gz
CRR151127_r2.fq.gz
|
11,951.69
12,453.19
|
|
Submitter | Yinchuan Li (18622397604@163.com) |
Organization | Nantong University |
Date submitted | 2020-07-15 |