Experiment information
Accession CRX125020
Organism Mus musculus
Title Dynamic profiles and transcriptional preferences of histone modifications during spermiogenesis
BioProject PRJCA003045
BioSample SAMC199710
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Single-cell suspensions with 1×10^5 cells/mL in concentration in PBS (HyClone) were prepared. Single-cell suspensions were then loaded onto microfluidic devices and scRNA-seq libraries were constructed according to Singleron GEXSCOPETM protocol by GEXSCOPETM Single-Cell RNA Library Kit (Singleron Biotechnologies) . Individual libraries were diluted to 4nM and pooled for sequencing. Pools were sequenced on Illumina HiSeq X with 150 bp paired end reads. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
Release date2020-07-15
Run
Run accession Run data file information
File nameFile size (MB)
CRR151127 CRR151127_f1.fq.gz
CRR151127_r2.fq.gz
11,951.69
12,453.19
SubmitterYinchuan Li (18622397604@163.com)
OrganizationNantong University
Date submitted2020-07-15
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