Experiment information
Accession CRX124947
Organism Sus scrofa
Title D66_1
BioProject PRJCA002917
BioSample SAMC195783
Platform Illumina MiSeq
Library
Library name Construction protocol Strategy Source Selection Layout
Using QIAamp DNA Stool Mini kit extraction piglet feces microbial genome DNA, The V3-V4 region of 16S rRNA gene was selected as the sequencing region, The specific primers (338F-806R) with a barcode are then synthesized, and the amplification was using the TransStart Fastpfu DNA Polymerase system. Then the Miseq libraries were constructed. Illumina Miseq platform was used for sequencing and the sequencing read length was PE300. OTHER GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 300
Planned read length (bp) for mate 2: 300
Insert size (bp): 468
Release date2021-02-12
Run
Run accession Run data file information
File nameFile size (MB)
CRR151054 CRR151054_f1.fq.gz
CRR151054_r2.fq.gz
5.15
7.02
SubmitterLi Diyan (diyanli@sicau.edu.cn)
OrganizationSichuan Agricultural University
Date submitted2020-07-14