Accession | CRX124940 |
Organism | Sus scrofa |
Title | H66_4 |
BioProject | PRJCA002917 |
BioSample | SAMC195776 |
Platform | Illumina MiSeq |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Using QIAamp DNA Stool Mini kit extraction piglet feces microbial genome DNA, The V3-V4 region of 16S rRNA gene was selected as the sequencing region, The specific primers (338F-806R) with a barcode are then synthesized, and the amplification was using the TransStart Fastpfu DNA Polymerase system. Then the Miseq libraries were constructed. Illumina Miseq platform was used for sequencing and the sequencing read length was PE300. |
OTHER |
GENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 300
Planned read length (bp) for mate 2: 300
Insert size (bp): 468
|
Release date | 2021-02-12 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR151047 |
CRR151047_f1.fq.gz
CRR151047_r2.fq.gz
|
5.43
7.71
|
|
Submitter | Li Diyan (diyanli@sicau.edu.cn) |
Organization | Sichuan Agricultural University |
Date submitted | 2020-07-14 |